Label free novel electrical detection using micromachined PZT monolithic thin film cantilever for the detection of C-reactive protein

被引：111

作者：

Lee, JH

Yoon, KH

Hwang, KS

Park, J

Ahn, S

Kim, TS

机构：

[1] KIST, Microsyst Res Ctr, Seoul 136791, South Korea

[2] Yonsei Univ, Dept Ceram Engn, Seoul 120749, South Korea

[3] Solco Biomed Co, Kyonggi Do 451852, South Korea

[4] Seoul Natl Univ, CTP, Seoul, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 20卷 / 02期

关键词：

nanomechanical biosensor; cantilever; PZT film; CRP; resonant frequency shift;

D O I：

10.1016/j.bios.2004.01.024

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, we report the novel electrical measurement for the label-free detection of C-reactive protein (CRP) using resonant frequency shift in the monolithic thin film cantilever of micromachined Pb(Zr0.52Ti0.48)O-3 (PZT) which was fabricated with the composition of SiO2/Ta/Pt/PZT/Pt/SiO2 on silicon nitride (SiNx) supporting layer for the dual purpose of electrical self-excitation and sensing. The specific binding characteristics of CRP antigen to its antibody, which is immobilized with Calixcrown SAMs on An surface deposited on microcantilever, is determined in high sensitivity to the nanogram level per milliliter by measuring the resonant frequency shift. The nanomechanical PZT cantilever turns out a robust platform for the highly specific antigen-antibody interaction and provides with the novel tool for qualification and quantification of biomolecules without any sample labeling and bulky optical apparatus. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：269 / 275

页数：7

共 24 条

[1] Development of nanomechanical biosensors for detection of the pesticide DDT [J].