Cyanobacterial circadian pacemaker: Kai protein complex dynamics in the KaiC phosphorylation cycle in vitro

被引：154

作者：

Kageyama, Hakuto

Nishiwaki, Taeko

Nakajima, Masato

Iwasaki, Hideo

Oyama, Tokitaka

Kondo, Takao

机构：

[1] Nagoya Univ, Div Biol Sci, Grad Sch Sci, Nagoya, Aichi 4648602, Japan

[2] Japan Sci & Technol Corp, CREST, Nagoya, Aichi 4648602, Japan

[3] Japan Sci & Technol Corp, SORST, Nagoya, Aichi 4648602, Japan

来源：

MOLECULAR CELL | 2006年 / 23卷 / 02期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/j.molcel.2006.05.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

KaiA, KaiB, and KaiC are essential proteins of the circadian clock in the cyanobacterium Synechococcus elongatus PCC 7942. The phosphorylation cycle of KaiC that occurs in vitro after mixing the three proteins and ATP is thought to be the master oscillation governing the circadian system. We analyzed the temporal profile of complexes formed between the three Kai proteins. In the phosphorylation phase, KaiA actively and repeatedly associated with KaiC to promote KaiC phosphorylation. High levels of phosphorylation of KaiC induced the association of the KaiC hexamer with KaiB and inactivate KaiA to begin the dephosphorylation phase, which is closely linked to shuffling of the monomeric KaiC subunits among the hexamer. By reducing KaiC phosphorylation, KaiB dissociated from KaiC, reactivating KaiA. We also confirmed that a similar model can be applied in cyanobacterial cells. The molecular model proposed here provides mechanisms for circadian timing systems.

引用

收藏

页码：161 / 171

页数：11

相关论文

共 24 条

[11] KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system [J].

Kitayama, Y ;

Iwasaki, H ;

Nishiwaki, T ;

Kondo, T .

EMBO JOURNAL, 2003, 22 (09) :2127-2134

[12] Circadian clock protein KaiC forms ATP-dependent hexameric rings and binds DNA [J].

Mori, T ;

Saveliev, SV ;

Xu, Y ;

Stafford, WF ;

Cox, MM ;

Inman, RB ;

Johnson, CH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :17203-17208

[13] Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro [J].

Nakajima, M ;

Imai, K ;

Ito, H ;

Nishiwaki, T ;

Murayama, Y ;

Iwasaki, H ;

Oyarna, T ;

Kondo, T .

SCIENCE, 2005, 308 (5720) :414-415

[14] Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942 [J].

Nishiwaki, T ;

Satomi, Y ;

Nakajima, M ;

Lee, C ;

Kiyohara, R ;

Kageyama, H ;

Kitayama, Y ;

Temamoto, M ;

Yamaguchi, A ;

Hijikata, A ;

Go, M ;

Iwasaki, H ;

Takao, T ;

Kondo, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (38) :13927-13932

[15] Visualizing a circadian clock protein: Crystal structure of KaiC and functional insights [J].

Pattanayek, R ;

Wang, JM ;

Mori, T ;

Xu, Y ;

Johnson, CH ;

Egli, M .

MOLECULAR CELL, 2004, 15 (03) :375-388

[16]

PITTENDRIGH CS, 1993, ANNU REV PHYSIOL, V55, P16

[17] No transcription-translation feedback in circadian rhythm of KaiC phosphorylation [J].

Tomita, J ;

Nakajima, M ;

Kondo, T ;

Iwasaki, H .

SCIENCE, 2005, 307 (5707) :251-254

[18] Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein [J].

Uzumaki, T ;

Fujita, M ;

Nakatsu, T ;

Hayashi, F ;

Shibata, H ;

Itoh, N ;

Kato, H ;

Ishiura, M .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (07) :623-631

[19] Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: Implications for KaiC regulation [J].

Vakonakis, I ;

LiWang, AC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (30) :10925-10930

[20] Structure and function from the circadian clock protein KaiA of Synechococcus elongatus:: A potential clock input mechanism [J].

Williams, SB ;

Vakonakis, I ;

Golden, SS ;

LiWang, AC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15357-15362