Cold-tolerant alkane-degrading Rhodococcus species from Antarctica

被引:94
作者
Bej, AK
Saul, D
Aislabie, J
机构
[1] Landcare Res, Hamilton, New Zealand
[2] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA
[3] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand
关键词
D O I
10.1007/s003000050014
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Bioremediation is a possible mechanism for clean-up of hydrocarbon-contaminated soils in the Antarctic. Microbes indigenous to the Antarctic are required that degrade the hydrocarbon contaminants found in the soil, and that are able to survive and maintain activity under in situ conditions. Alkane-degrading bacteria previously isolated from oil-contaminated soil from around Scott Base, Antarctica, grew on a number of n-alkanes from hexane (C6) through to eicosane (C20) and the branched alkane pristane. Mineralization of C-14-dodecane was demonstrated with four strains. Representative isolates were identified as Rhodococcus species using 16S rDNA sequence analysis. Rhodococcus spp. strains 5/14 and 7/1 grew at -2 degrees C but numbers of viable cells declined when incubated at 37 degrees C. Both strains appear to have the major cold-shock gene cspA. Partial nucleotide sequence analyses of the PCR-amplified cspA open reading frame from Rhodococcus spp. strains 5/14 and 7/1 were approximately 60% identical to cspA from Escherichia coli.
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页码:100 / 105
页数:6
相关论文
共 23 条
[11]   Growth, survival and characterization of cspA in Salmonella enteritidis following cold shock [J].
Jeffreys, AG ;
Hak, KM ;
Steffan, RJ ;
Foster, JW ;
Bej, AK .
CURRENT MICROBIOLOGY, 1998, 36 (01) :29-35
[12]   Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli [J].
Jones, PG ;
Mitta, M ;
Kim, Y ;
Jiang, WN ;
Inouye, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) :76-80
[13]  
KERRY E, 1990, POLAR BIOL, V10, P423, DOI 10.1007/BF00233690
[14]   Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium [J].
MacCormack, WP ;
Fraile, ER .
ANTARCTIC SCIENCE, 1997, 9 (02) :150-155
[15]   SOLUTION NMR STRUCTURE OF THE MAJOR COLD SHOCK PROTEIN (CSPA) FROM ESCHERICHIA-COLI - IDENTIFICATION OF A BINDING EPITOPE FOR DNA [J].
NEWKIRK, K ;
FENG, WQ ;
JIANG, WN ;
TEJERO, R ;
EMERSON, SD ;
INOUYE, M ;
MONTELIONE, GT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :5114-5118
[16]   Short communication: Production and properties of a biosurfactant synthesized by Arthrobacter protophormiae - An Antarctic strain [J].
Pruthi, V ;
Cameotra, SS .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1997, 13 (01) :137-139
[17]   PHYLOGENETIC ANALYSIS OF THE GENERA RHODOCOCCUS AND NOCARDIA AND EVIDENCE FOR THE EVOLUTIONARY ORIGIN OF THE GENUS NOCARDIA FROM WITHIN THE RADIATION OF RHODOCOCCUS SPECIES [J].
RAINEY, FA ;
BURGHARDT, J ;
KROPPENSTEDT, RM ;
KLATTE, S ;
STACKEBRANDT, E .
MICROBIOLOGY-UK, 1995, 141 :523-528
[18]   OCCURRENCE AND EXPRESSION OF CSPA, A COLD SHOCK GENE, IN ANTARCTIC PSYCHROTROPHIC BACTERIA [J].
RAY, MK ;
SITARAMAMMA, T ;
GHANDHI, S ;
SHIVAJI, S .
FEMS MICROBIOLOGY LETTERS, 1994, 116 (01) :55-60
[19]   PHYLOGENY OF 20 THERMUS ISOLATES CONSTRUCTED FROM 16S RIBOSOMAL-RNA GENE SEQUENCE DATA [J].
SAUL, DJ ;
RODRIGO, AG ;
REEVES, RA ;
WILLIAMS, LC ;
BORGES, KM ;
MORGAN, HW ;
BERGQUIST, PL .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (04) :754-760
[20]  
Thieringer HA, 1998, BIOESSAYS, V20, P49