Copper proteins immobilised on gold electrodes for (bio)analytical studies

被引：20

作者：

Lisdat, F ^{[1
]}

Karube, I ^{[1
]}

机构：

[1] Univ Tokyo, Ctr Collaborat Res, Tokyo 15308904, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 11-12期

关键词：

copper protein; azurin; metallothionein; gold electrode; oxidative stress; copper detection;

D O I：

10.1016/S0956-5663(02)00097-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Copper electrochemistry at modified gold electrodes was investigated with two different states of the metal ion: first bound in azurin from Pseudomonas aeruginosa and second introduced via metal ion uptake in metallothionein (NIT) from rabbit liver. Azurin was immobilised on a mercaptosuccinic acid (MSA) layer self-assembled on gold. The redox behaviour in the adsorbed as well as in the covalently immobilised state was found to be quasi-reversible with a formal potential of + 198 mV versus Ag/AgCl. The pH variation suggests an optimal pH range for efficient electrode communication in the neutral range. NIT was fixed at electrochemically cleaned gold using the accessible cysteins of the protein. Copper was found to bind to the MT-modified gold electrode. The electrochemical behaviour of the bound copper was characterised in copper-free solution with a formal potential of + 245 mV versus Ag/AgCl. Stability and potential use is discussed. (C) 2002 Published by Elsevier Science B.V.

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页码：1051 / 1057

页数：7

相关论文

共 35 条

[1] Fast voltammetric studies of the kinetics and energetics of coupled electron-transfer reactions in proteins [J].

Armstrong, FA ;

Camba, R ;

Heering, HA ;

Hirst, J ;

Jeuken, LJC ;

Jones, AK ;

Léger, C ;

McEvoy, JP .

FARADAY DISCUSSIONS, 2000, 116 :191-203

[2] High resolution solution structure of the protein part of Cu7 metallothionein [J].

Bertini, I ;

Hartmann, HJ ;

Klein, T ;

Liu, GH ;

Luchinat, C ;

Weser, U .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (04) :1008-1018

[3] Molecular monolayers and interfacial electron transfer of Pseudomonas aeruginosa azurin on Au(111) [J].

Chi, QJ ;

Zhang, JD ;

Nielsen, JU ;

Friis, EP ;

Chorkendorff, I ;

Canters, GW ;

Andersen, JET ;

Ulstrup, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (17) :4047-4055

[4] Whole cell- and protein-based biosensors for the detection of bioavailable heavy metals in environmental samples [J].

Corbisier, P ;

van der Lelie, D ;

Borremans, B ;

Provoost, A ;

de Lorenzo, V ;

Brown, NL ;

Lloyd, JR ;

Hobman, JL ;

Csöregi, E ;

Johansson, G ;

Mattiasson, B .

ANALYTICA CHIMICA ACTA, 1999, 387 (03) :235-244

[5] Electrochemical study of human foetal liver metallothionein: influence of the additions of cadmium and zinc [J].

Dabrio, M ;

Rodriguez, AR .

ANALYTICA CHIMICA ACTA, 2000, 406 (02) :171-181

[6] Protein adsorption at a gold electrode studied by in situ scanning tunnelling microscopy [J].

Davis, JJ ;

Halliwell, CM ;

Hill, HAO ;

Canters, GW ;

van Amsterdam, MC ;

Verbeet, MP .

NEW JOURNAL OF CHEMISTRY, 1998, 22 (10) :1119-1123

[7] A reagentless nitric oxide biosensor based on hemoglobin-DNA films [J].

Fan, CH ;

Li, GX ;

Zhu, JQ ;

Zhu, DX .

ANALYTICA CHIMICA ACTA, 2000, 423 (01) :95-100

[8] Measurement of electron transfer rates between adsorbed azurin and a gold electrode modified with a hexanethiol layer [J].

Gaigalas, AK ;

Niaura, G .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1997, 193 (01) :60-70

[9]

GE B, 2001, IN PRESS BIOSENS BIO

[10] Efficient mediatorless superoxide sensors using cytochrome c-modified electrodes:: surface nano-organization for selectivity and controlled peroxidase activity [J].

Gobi, KV ;

Mizutani, F .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2000, 484 (02) :172-181

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