A method for linking yeast artificial chromosomes

被引：9

作者：

Larin, Z ^{[1
]}

Taylor, SS ^{[1
]}

TylerSmith, C ^{[1
]}

机构：

[1] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/24.21.4192

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the beta-galactosidase gene as an expression reporter in mammalian cells.

引用

收藏

页码：4192 / 4196

页数：5

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