Mosaic DNA Imports with Interspersions of Recipient Sequence after Natural Transformation of Helicobacter pylori

被引:32
作者
Kulick, Stefan [1 ]
Moccia, Claudia [1 ]
Didelot, Xavier [2 ]
Falush, Daniel [3 ]
Kraft, Christian [1 ]
Suerbaum, Sebastian [1 ]
机构
[1] Hannover Med Sch, Inst Med Microbiol, D-3000 Hannover, Germany
[2] Univ Warwick, Dept Stat, Coventry CV4 7AL, W Midlands, England
[3] Univ Coll Cork, Environm Res Inst, Cork, Ireland
来源
PLOS ONE | 2008年 / 3卷 / 11期
关键词
D O I
10.1371/journal.pone.0003797
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Helicobacter pylori colonizes the gastric mucosa of half of the human population, causing gastritis, ulcers, and cancer. H. pylori is naturally competent for transformation by exogenous DNA, and recombination during mixed infections of one stomach with multiple H. pylori strains generates extensive allelic diversity. We developed an in vitro transformation protocol to study genomic imports after natural transformation of H. pylori. The mean length of imported fragments was dependent on the combination of donor and recipient strain and varied between 1294 bp and 3853 bp. In about 10% of recombinant clones, the imported fragments of donor DNA were interrupted by short interspersed sequences of the recipient (ISR) with a mean length of 82 bp. 18 candidate genes were inactivated in order to identify genes involved in the control of import length and generation of ISR. Inactivation of the antimutator glycosylase MutY increased the length of imports, but did not have a significant effect on ISR frequency. Overexpression of mutY strongly increased the frequency of ISR, indicating that MutY, while not indispensable for ISR formation, is part of at least one ISR-generating pathway. The formation of ISR in H. pylori increases allelic diversity, and contributes to the uniquely low linkage disequilibrium characteristic of this pathogen.
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页数:9
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