Determination of flavonoids in a Citrus fruit extract by LC-DAD and LC-MS

被引:92
作者
de Lourdes Mata Bilbao, Maria
Andres-Lacueva, Cristina
Jauregui, Olga
Lamuela-Raventos, Rosa Maria
机构
[1] Univ Barcelona, Fac Pharm, Dept Nutr & Bromotol, CerTA, E-08028 Barcelona, Spain
[2] Univ Barcelona, Fac Pharm, Sci & Tech Serv, CerTA, E-08028 Barcelona, Spain
关键词
flavonoids; Citrus; chromatography; mass spectrometry;
D O I
10.1016/j.foodchem.2006.01.032
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Flavonoids are a group of polyphenolic compounds with health-related properties. Citrus fruits are rich in flavonoids and their extracts are being used as functional ingredients for several industrial products. A new high performance liquid chromatography technique with an UV photodiode-array detector was used to analyze flavonoids of an extract of Citrus species. To our knowledge this is the first study that reports isoquercitrin presence at a level of 77.3 mg/100 g in a sample made of Citrus fruits; four other flavonoids were quantified as rutin (326.59 mg/100 g), naringin (338.36 mg/100 g), quercetin (96.35 mg/100 g) and naringenin (2.35 mg/100 g). Identification was confirmed by a liquid chromatography mass spectrometer system. Method validation was achieved, providing an analytical technique that can be used to detect trace amounts of these compounds in Citrus extracts with an extremely rapid sample preparation. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1742 / 1747
页数:6
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