Determination of ephedra alkaloid and caffeine concentrations in dietary supplements and biological fluids

被引：28

作者：

Jacob, P ^{[1
]}

Haller, CA ^{[1
]}

Duan, MJ ^{[1
]}

Yu, L ^{[1
]}

Peng, M ^{[1
]}

Benowitz, NL ^{[1
]}

机构：

[1] Univ Calif San Francisco, Div Clin Pharmacol, San Francisco Gen Hosp, Dept Med, San Francisco, CA 94110 USA

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2004年 / 28卷 / 03期

关键词：

D O I：

10.1093/jat/28.3.152

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Dietary supplements containing botanical forms of caffeine and ephedra alkaloids have been widely promoted and used in the U.S. for weight loss and athletic enhancement despite a lack of adequate research on the pharmacology of these botanical stimulants. In order to analyze dietary supplements and perform human pharmacokinetic studies, an analytical approach with good precision and accuracy was needed with sufficient sensitivity to detect very low levels of ephedra alkaloids. A liquid chromatography-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (LC-MS-MS) method was developed for quantitating the various ephedrine-group alkaloids found in dietary supplements that contain Ephedra species, and in plasma and urine of persons consuming these supplements. Using this method, low nanogram-per-milliliter concentrations of ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine, methylpseudoephedrine, and caffeine can be quantitated in a 12-min LC-MS-MS run.

引用

页码：152 / 159

页数：8

共 14 条

[1] Sensitive determination of ephedrine and norephedrine in human plasma samples using derivatization with 9-fluorenylmethyl chloroformate and liquid chromatography [J].