Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and α-amylase

被引：142

作者：

Shigechi, H

Koh, J

Fujita, Y

Matsumoto, T

Bito, Y

Ueda, M

Satoh, E

Fukuda, H

Kondo, A

机构：

[1] Kobe Univ, Dept Chem Sci & Engn, Fac Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Div Mol Sci, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Kyoto Univ, Dept Appl Biochem, Div Appl Life Sci, Grad Sch Agr,Sakyo Ku, Kyoto 6068502, Japan

[4] Tokyo Univ Agr, Dept Appl Biol & Chem, Setagaya Ku, Tokyo 1568502, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 08期

关键词：

D O I：

10.1128/AEM.70.8.5037-5040.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis a-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.

引用

收藏

页码：5037 / 5040

页数：4

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