Application of Convective Interaction Media disks with immobilised glucose oxidase for on-line glucose measurements

被引：40

作者：

Vodopivec, M

Berovic, M

Jancar, J

Podgornik, A

Strancar, A

机构：

[1] Natl Inst Chem, Ljubljana 1000, Slovenia

[2] BIA Seperat DOO, Ljubljana 1000, Slovenia

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 407卷 / 1-2期

关键词：

convective interaction media; glucose oxidase immobilisation; enzyme reactor; fermentation processes;

D O I：

10.1016/S0003-2670(99)00817-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new polymeric macroporous material, Convective Interaction Media (CIM) was applied as a support for glucose oxidase (GOD) immobilisation. CIM epoxy disks with the immobilised enzyme were integrated as an enzyme reactor in a flow injection analysis (FIA) system and applied to on-line monitoring of glucose during cultivation of Saccharomyces cerevisiae and citric acid production by Aspergillus niger. The developed CIM GOD disk-FIA system exhibited good signal reproducibility and satisfactory long-term stability with a linear response in the range 10-200 mg 1(-1). The CIM disk applied as an enzyme reactor proved to be a successful replacement for conventionally used packed-bed columns and as such it was well suited for on-line monitoring of bioprocesses. (C)2000 Elsevier Science B.V. All rights reserved.

引用

页码：105 / 110

页数：6

共 19 条

[1] BIOSENSORS FOR PROCESS-CONTROL [J].