DEVELOPMENT OF ENZYME CARTRIDGE FLOW-INJECTION ANALYSIS FOR INDUSTRIAL-PROCESS MONITORING .2. APPLICATION FOR MONITORING OF MICROORGANISM CULTIVATIONS

被引：16

作者：

JURGENS, H

BRANDES, L

JOPPIEN, R

SIEBOLD, M

SCHUBERT, J

WU, X

KRETZMER, G

SCHUGERL, K

机构：

[1] UNIV HANNOVER,INST TECH CHEM,D-30167 HANNOVER,GERMANY

[2] EPPENDORF NETHELER HINZ,HAMBURG,GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 302卷 / 2-3期

关键词：

FLOW INJECTION; ENZYMATIC METHODS; PROCESS ANALYSIS; MICROORGANISMS;

D O I：

10.1016/0003-2670(94)00492-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Five cartridges with immobilized glucose oxydase, alcohol oxydase, pyranose oxydase, L-amino acid oxydase and L-lactate-2-monooxigenase, respectively, were integrated into a flow injection analysis (FIA) system and applied to the monitoring of medium components: glucose and ethanol during the cultivation of Saccharomyces cerevisiae, L-amino acids during the production of the fusion protein EcoRI::SPA by recombinant Escherichia coli, and lactate during the production of lactic acid by Lactobacillus salivarius var. salivarius. The developed enzyme cartridge-FIA systems show a reproducible operation, low pressure drop and long-range enzyme activities and are well suited for on-line monitoring of microbial cultivation processes.

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页码：289 / 296

页数：8

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