PRODUCTION OF FUSION PROTEIN SPA - ECORI IN BATCH CULTURE IN A 60-L AIRLIFT TOWER LOOP REACTOR

被引:10
作者
BRANDES, L
WU, XO
MASCHKE, HE
JURGENS, H
REINHARDT, B
SCHUGERL, K
机构
[1] UNIV HANNOVER, INST TECH CHEM, CALLINSTR 3, W-3000 HANNOVER, GERMANY
[2] GENENTECH INC, San Francisco, CA 94080 USA
[3] INST MED MEMBRANANWENDUNGEN, AKZO, W-5600 WUPPERTAL 2, GERMANY
关键词
D O I
10.1021/bp00020a002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Escherichia coli JM103 carrying the expression plasmid pMTC48, repressor plasmid pRK248, and protection plasmid pEcoR4 was grown in a 60-L working volume airlift tower loop reactor on M9 minimal medium. Production of fusion protein SpA::EcoRI was induced by a temperature shift from 30 to 38 (optimum),40, or 42-degrees-C. The following parameters were monitored: cell mass concentration (X), total cell counts (TCC), number of colony-forming units (CFU), concentrations of glucose, acetate, ethanol, pyruvate, lactate, succinate, amino acids, and ammonia, and soluble and total protein content, as well as product concentration (enzyme activity of the fusion protein), dissolved oxygen concentration, oxygen utilization rate (OUR), CO2 production rate (CPR), respiration quotient (RQ), and volumetric mass-transfer coefficients (k(L)a). Product formation by temperature shift was only observed if LB concentrate was added to the culture at the same time the aeration rate was increased to avoid oxygen-transfer limitation. No product accumulation was observed with glucose and ammonia supplementation. During gene expression, X and TCC increased, CFU decreased, acetate increased, and the primary metabolite (ethanol, pyruvate, lactate) concentrations as well as OUR and CPR passed a maximum while RQ changed only slightly. These facts indicate that, during gene expression, the metabolic activity of the cell passes a maximum, and after that it decreases. With increasing aeration rate, the volumetric productivity increased, but the specific productivity with respect to the cell concentration decreased.
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页码:122 / 127
页数:6
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