Control of column temperature in reversed-phase liquid chromatography

被引：90

作者：

Wolcott, RG

Dolan, JW

Snyder, LR

Bakalyar, SR

Arnold, MA

Nichols, JA

机构：

[1] LC Resources Inc, Walnut Creek, CA 94596 USA

[2] Linfield Coll, Dept Chem, McMinnville, OR 97128 USA

[3] Rheodyne LP, Rohnert Park, CA 94927 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 869卷 / 1-2期

关键词：

column temperature; stationary phase; LC;

D O I：

10.1016/S0021-9673(99)00894-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

When separations by reversed-phase liquid chromatography (RP-LC) are carried out at temperatures other than ambient, resulting retention times and bandwidths can depend on the equipment used. As a result, an RP-LC separation that is adequate when carried out on one LC system may prove inadequate when the separation is repeated on a second system. In the present study, various temperature-related problems which can result in a failure of method transfer for non-ambient RP-LC methods were examined. Means for correcting for such effects and thereby ensuring method transferability are described. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：211 / 230

页数：20

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