Micropatterned agarose gels for stamping arrays of proteins and gradients of proteins

被引：111

作者：

Mayer, M ^{[1
]}

Yang, J ^{[1
]}

Gitlin, I ^{[1
]}

Gracias, DH ^{[1
]}

Whitesides, GM ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

PROTEOMICS | 2004年 / 4卷 / 08期

关键词：

hydrogel; micropatterning; protein gradients; protein microarrays; stamping;

D O I：

10.1002/pmic.200300748

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for repetitive and rapid formation of planar microarrays and gradients of proteins using patterned agarose stamps. It demonstrates: (i) micropatterning of agarose gels with feature sizes as small as 2 mum; (ii) inking of posts (diameter 50-1000 mum) on patterned agarose stamps with one or multiple (here, eight) proteins and repetitive stamping of patterns (>100 times in the case of one protein) and arrays (20 times in the case of eight proteins) without the need for intermediate re-inking; (iii) transferring spots of proteins with good homogeneity in surface coverage to glass slides; (iv) applying this technique to surface-based immunoassays; (v) stamping that requires only sub-nanomolar amounts of protein (typically -3 mug in g0.6 mug of solution); (vi) stamping without the need for drying of the proteins, as opposed to stamping with stamps made of poly(dimethylsiloxane); and (vii) patterning gradients of proteins by allowing two proteins to diffuse toward each other in an agarose stamp, followed by printing the protein gradients onto a surface.

引用

页码：2366 / 2376

页数：11