A doubly amplified electrochemical immunoassay for carcinoembryonic antigen

被引：9

作者：

Gao, Zhiqiang ^{[1
]}

Zhang, Jie ^{[1
]}

Ting, Boon Ping ^{[1
]}

机构：

[1] Inst Bioengn & Nanotechnol, Singapore 138669, Singapore

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 07期

关键词：

Immunoassay; Carcinoembryonic antigen; Amperometry; Redox polymer; Glucose oxidase; LINKED-IMMUNOSORBENT-ASSAY; PROTEIN MICROARRAYS; WHOLE-BLOOD; ENZYMES; ACID; DNA;

D O I：

10.1016/j.bios.2008.09.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An ultrasensitive electrochemical immunoassay(EIA) for the detection of carcinoembryonic antigen (CEA) is described in this report. The assay involves utilizing enzyme-catalyzed deposition of a redox polymer and electrocatalytic oxidation of ascorbic acid (AA) by the deposited redox polymer, a dual-amplification scheme to enhance analytical signals. Briefly, CEA capturing antibody and redox polymer anchoring agent were covalently immobilized on a gold electrode. After incubating with CEA, the electrode was treated in detection antibody-glucose oxidase conjugate solution. Thereafter, it was dipped into the redox polymer solution. Upon the addition of glucose, the redox polymer was enzymatically reduced and deposited on the electrode surface. The deposited redox polymer exhibits excellent electrocatalytic activity towards the oxidation of AA. Consequently, CEA could be quantified amperometrically. This electrochemical immunoassay combines the specificity of the immunological reaction with the sensitivity of the doubly amplified electrochemical detection. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1825 / 1830

页数：6

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