CFTR is a monomer: Biochemical and functional evidence

被引:30
作者
Chen, JH [1 ]
Chang, XB [1 ]
Aleksandrov, AA [1 ]
Riordan, JR [1 ]
机构
[1] Mayo Clin & Mayo Fdn, SC Johnson Med Res Ctr, Scottsdale, AZ 85259 USA
关键词
ABC protein; CFTR; chloride channel; cystic fibrosis; quaternary structure; CFTR monomer;
D O I
10.1007/s00232-001-0174-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although the CFTR protein alone is sufficient to generate a regulated chloride channel, it is unknown how many of the polypeptides form the channel. Using biochemical and functional assays, we demonstrate that the CFTR polypeptide is a monomer. CFTR sediments as a monomer in a linear, continuous sucrose gradient. Cells co-expressing different epitope-tagged CFTR provide no evidence of co-assembly in immunoprecipitation and nickel affinity binding experiments. Co-expressed wild-type and DeltaF508 CFTR are without influence on each other in their ability to progress through the secretory pathway, suggesting they do not associate in the endoplasmic reticulum. No hybrid conducting single channels are seen in planar lipid bilayers with which membrane vesicles from cells co-expressing similar amounts of two different CFTR conduction species have been fused.
引用
收藏
页码:55 / 71
页数:17
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