Quantification of metabolites of the nerve agents sarin, soman, cyclohexylsarin, VX, and Russian VX in human urine using isotope-dilution gas chromatography-tandem mass spectroscopy

被引：50

作者：

Barr, JR

Driskell, WJ

Aston, LS

Martinez, RA

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, DLS, ERAT, Atlanta, GA 30341 USA

[2] Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, Battelle Mem Inst, Atlanta, GA 30341 USA

[3] Natl Stable Isotope Resource Los Alamos Natl Lab, Los Alamos, NM USA

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2004年 / 28卷 / 05期

关键词：

D O I：

10.1093/jat/28.5.372

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Organophosphorus nerve agents are among the most toxic organic compounds known and continue to be a threat for both military and terrorist use. We have developed an isotope-dilution gas chromatography-tandem mass spectrometric (GC-MS-MS) method for quantitating the urinary metabolites of the organophosphorus nerve agents sarin (GB), soman (GD), VX, Russian VX (RVX), and cyclohexylsarin (GF). Urine samples were acidified, extracted into ether-acetonitrile, derivatized by methylation with diazomethane, and analyzed by GC-MS-MS. The limits of detection were less than 1 μg/L for all analytes.

引用

收藏

页码：372 / 378

页数：7

相关论文

共 20 条

[1] SYNTHESIS OF [C-14] METHYLPHOSPHONIC DIFLUORIDE [J].

BECHTOLD, WE ;

DAHL, AR .

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1985, 22 (11) :1181-1186

[2] APPLICATION OF GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY TO THE ANALYSIS OF CHEMICAL WARFARE SAMPLES, FOUND TO CONTAIN RESIDUES OF THE NERVE AGENT SARIN, SULFUR MUSTARD AND THEIR DEGRADATION PRODUCTS [J].

BLACK, RM ;

CLARKE, RJ ;

READ, RW ;

REID, MTJ .

JOURNAL OF CHROMATOGRAPHY A, 1994, 662 (02) :301-321

[3] Quantitation of organophosphorus nerve agent metabolites in human urine using isotope dilution gas chromatography tandem mass spectrometry [J].

Driskell, WJ ;

Shih, M ;

Needham, LL ;

Barr, DB .

JOURNAL OF ANALYTICAL TOXICOLOGY, 2002, 26 (01) :6-10

[4] TRACE DETERMINATION OF ALKYL METHYLPHOSPHONIC ACIDS IN ENVIRONMENTAL AND BIOLOGICAL SAMPLES USING GAS-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY [J].

FREDRIKSSON, SA ;

HAMMARSTROM, LG ;

HENRIKSSON, L ;

LAKSO, HA .

JOURNAL OF MASS SPECTROMETRY, 1995, 30 (08) :1133-1143

[5] Determination of the main hydrolysis products of organophosphorus nerve agents, methylphosphonic acids, in human serum by indirect photometric detection ion chromatography [J].

Katagi, M ;

Nishikawa, M ;

Tatsuno, M ;

Tsuchihashi, H .

JOURNAL OF CHROMATOGRAPHY B, 1997, 698 (1-2) :81-88

[6] On-line solid-phase extraction liquid chromatography-continuous flow frit fast atom bombardment mass spectrometric and tandem mass spectrometric determination of hydrolysis products of nerve agents alkyl methylphosphonic acids by p-bromophenacyl derivatization [J].

Katagi, M ;

Tatsuno, M ;

Nishikawa, M ;

Tsuchihashi, H .

JOURNAL OF CHROMATOGRAPHY A, 1999, 833 (02) :169-179

[7] PRINCIPLES OF ENVIRONMENTAL-ANALYSIS [J].

KEITH, LH ;

CRUMMETT, W ;

DEEGAN, J ;

LIBBY, RA ;

TAYLOR, JK ;

WENTLER, G .

ANALYTICAL CHEMISTRY, 1983, 55 (14) :2210-2218

[8] ONLINE FLAME PHOTOMETRIC DETECTION IN MICRO-COLUMN LIQUID-CHROMATOGRAPHY [J].

KIENTZ, CE ;

VERWEIJ, A ;

BOTER, HL ;

POPPEMA, A ;

FREI, RW ;

DEJONG, GJ ;

BRINKMAN, UAT .

JOURNAL OF CHROMATOGRAPHY, 1989, 467 (02) :385-394

[9] ION-CHROMATOGRAPHIC SEPARATION OF CLOSELY-RELATED NERVE AGENT DEGRADATION PRODUCTS USING AN ORGANIC MODIFIER TO PROVIDE SELECTIVITY [J].

KINGERY, AF ;

ALLEN, HE .

ANALYTICAL CHEMISTRY, 1994, 66 (01) :155-159

[10] Determination of alkylmethylphosphonic acids, the main metabolites of organophosphorus nerve agents, in biofluids by gas chromatography mass spectrometry and liquid-liquid-solid-phase-transfer-catalyzed pentafluorobenzylation [J].

Miki, A ;

Katagi, M ;

Tsuchihashi, H ;

Yamashita, M .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1999, 23 (02) :86-93