Protective effect of oren-gedoku-to against induction of neuronal death by transient cerebral ischemia in the C57BL/6 mouse

被引：47

作者：

Kondo, Y ^{[1
]}

Kondo, F ^{[1
]}

Asanuma, M ^{[1
]}

Tanaka, K ^{[1
]}

Ogawa, N ^{[1
]}

机构：

[1] Okayama Univ, Sch Med, Dept Neurosci, Inst Mol & Cellular Med, Okayama 7008558, Japan

来源：

NEUROCHEMICAL RESEARCH | 2000年 / 25卷 / 02期

关键词：

TJ-15; transient forebrain ischemia; C57BL/6; mice; Cu/Zn-SOD;

D O I：

10.1023/A:1007515318434

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the neuroprotective effects of oren-gedoku-to (TJ15), a herbal medicine, after transient forebrain ischemia. Transient forebrain ischemia was induced by occlusion of both common carotid arteries for 15 min in C57BL/6 mice treated with TJ15. In the control ischemic group without TJ15 treatment, histologic examination of brain tissue collected seven days after reperfusion showed death of pyramidal cells in CA2-3 area of the hippocampus, unilaterally or bilaterally. In mice treated with oral TJ15 (845 mg/kg/day) for five weeks, the frequency of ischemic neuronal death was significantly lower. Immunohistochemistry for Cu/Zn-superoxide dismutase (Cu/Zn-SOD) showed strongly reactive astrocytes in the hippocampus of ischemic mice treated with TJ15. Damage to nerve cells by free radicals plays an important role in the induction of neuronal death by ischemia-reperfusion injury. Our results suggest that TJ15 protects against ischemic neuronal death by increasing the expression of Cu/Zn-SOD and suggest that oren-gedoku-to reduces the exposure of hippocampal neurons to oxidative stress.

引用

页码：205 / 209

页数：5

共 23 条

[21]

Wu W, 1992, J Tradit Chin Med, V12, P183

[22] C57BL/6 strain is most susceptible to cerebral ischemia following bilateral common carotid occlusion among seven mouse strains: Selective neuronal death in the murine transient forebrain ischemia [J].

Yang, GM ;

Kitagawa, K ;

Matsushita, K ;

Mabuchi, T ;

Yagita, Y ;

Yanagihara, T ;

Matsumoto, M .

BRAIN RESEARCH, 1997, 752 (1-2) :209-218

[23] HUMAN COPPER-ZINC SUPEROXIDE-DISMUTASE TRANSGENIC MICE ARE HIGHLY RESISTANT TO REPERFUSION INJURY AFTER FOCAL CEREBRAL-ISCHEMIA [J].

YANG, GY ;

CHAN, PH ;

CHEN, J ;

CARLSON, E ;

CHEN, SF ;

EPSTEIN, P ;

KAMII, H .

STROKE, 1994, 25 (01) :165-170

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