Indirect time-resolved fluorescence detection of both non-fluorescent and fluorescent compounds separated by high-performance liquid chromatography

被引：3

作者：

Kurosu, Y ^{[1
]}

Iwata, T ^{[1
]}

Tsuji, A ^{[1
]}

Maeda, M ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,SHINAGAWA KU,TOKYO 142,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 787卷 / 1-2期

关键词：

indirect time-resolved fluorescence detection; detection; LC; derivatization; phenylthiohydantoin-alanine; dansyl-alanine; Europium chelates;

D O I：

10.1016/S0021-9673(97)00622-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Indirect time-resolved fluorescence (ITRF) detection of non-fluorescent phenylthiohydantoin (PTH)-Ala and fluorescent dansyl (DNS)-Ala separated by high-performance liquid chromatography (HPLC) was carried out. Addition of europium (Eu3+) chelates to the eluent makes it possible to detect both non-fluorescent and fluorescent compounds simultaneously. This is because the fluorescence lifetime of Eu3+ chelates is extremely long, whereas that of normal fluorescent compounds is short. The HPLC conditions with the Eu3+ chelate system were studied. (C) 1997 Elsevier Science B.V.

引用

页码：261 / 265

页数：5

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