Construction of time-resolved fluorescence detector for amino compounds after high-performance liquid chromatography using europium chelate

被引：19

作者：

Iwata, T ^{[1
]}

Senda, M ^{[1
]}

Kurosu, Y ^{[1
]}

Tsuji, A ^{[1
]}

Maeda, M ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,SINAGAWA KU,TOKYO 142,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 10期

关键词：

D O I：

10.1021/ac961224w

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have constructed a simple, low-cost, time-resolved fluorescence detector (TRFD) for analysis of amino compounds with high-performance liquid chromatography using europium (Eu3+) chelates, which consists of a dc-operated xenon lamp, two synchronized mechanical choppers, and spectroscopic filters. Amino compounds derivatized by isothiocyanobenzyl EDTA with Eu3+ chelate are mixed with an enhancer solution postcolumn and detected by the TRFD. Taking advantage of the long fluorescence lifetime of the Eu3+ chelates, high selectivity against background fluorecein is achieved. To demonstrate the usefulness of the TRFD, fundamental performance test comparison with the conventional fluorescence detector was carried out. Details of the system are also described.

引用

页码：1861 / 1865

页数：5

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