High-level production of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) in high-density cultivation of recombinant Escherichia coli using a combined feeding strategy

被引：34

作者：

Shen, YL ^{[1
]}

Zhang, Y ^{[1
]}

Sun, AY ^{[1
]}

Xia, XX ^{[1
]}

Wei, DZ ^{[1
]}

Yang, SL ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 12期

基金：

国家高技术研究发展计划(863计划);

关键词：

combined feeding strategy; Escherichia coli; fed-batch; high-cell-density cultivation; TRAIL;

D O I：

10.1023/B:BILE.0000030043.84226.ac

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant Escherichia coli strain C600/pBV-TRAIL (encoding for 114-281 amino acids of TRAIL's soluble fragment) produced a recombinant human tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Using a combined strategy of exponential feeding and pH-stat feeding, high concentrations of biomass (65 g dry wt l(-1)) and active soluble TRAIL (1.4 g l(-1)) were obtained within 30 h. The accumulation of acetate, which usually occurs during the process of high-density culture of Escherichia coli and especially in the induction stage of protein synthesis, was avoided.

引用

页码：981 / 984

页数：4

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