Heterologous Kluyveromyces lactis beta-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants

被引：23

作者：

Becerra, M ^{[1
]}

Cerdan, E ^{[1
]}

Siso, MIG ^{[1
]}

机构：

[1] FAC CIENCIAS, DEPT BIOL CELULAR & MOL, LA CORUNA 15071, SPAIN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1997年 / 1335卷 / 03期

关键词：

beta-galactosidase; yeast autolytic mutant;

D O I：

10.1016/S0304-4165(97)00048-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The beta-galactosidase from Kluyveromyces lactis is a high molecular weight protein with commercial interest. A major drawback of its industrial production is the high cost associated with extraction and downstream processing due to its intracellular nature. In this work, the effectiveness of the utilization of Saccharomyces cerevisiae LD1 and LHDP1 strains, osmotic-remedial mutants which lyse at 37 degrees C, for the heterologous production and release into the extracellular medium of this protein has been proved. The highest absolute values of released beta-galactosidase have been obtained with the protease-deficient strain LHDP1 by osmotic shock.

引用

页码：235 / 241

页数：7

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