An Alternative DNA Structure Is Necessary for Pilin Antigenic Variation in Neisseria gonorrhoeae

被引：240

作者：

Cahoon, Laty A. ^{[1
]}

Seifert, H. Steven ^{[1
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA

来源：

SCIENCE | 2009年 / 325卷 / 5941期

关键词：

HOMOLOGOUS RECOMBINATION; G-QUADRUPLEX; NUCLEOTIDE-SEQUENCES; G4; DNA; GENE; REPAIR; PATHWAYS; PROTEIN; PATHOGENS; BACTERIAL;

D O I：

10.1126/science.1175653

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pathogens can use DNA recombination to promote antigenic variation (Av) of surface structures to avoid immune detection. We identified a cis-acting DNA sequence near the antigenically variable pilin locus of the human pathogen, Neisseria gonorrhoeae. This 16-base pair guanine (G)-rich sequence was required for pilin Av and formed a guanine quartet (G4) structure in vitro. Individual mutations that disrupted the structure also blocked pilin Av and prevented nicks required for recombination from occurring within the G4 region. A compound that binds and stabilizes G4 structures also inhibited pilin Av and prevented nicks from occurring on the G-rich strand. This site constitutes a recombination initiation sequence/structure that directs gene conversion to a specific chromosomal locus.

引用

页码：764 / 767

页数：4

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