Silencing of the tapetum-specific zinc finger gene TAZ1 causes premature degeneration of tapetum and pollen abortion in petunia

被引：129

作者：

Kapoor, S ^{[1
]}

Kobayashi, A ^{[1
]}

Takatsuji, H ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Dev Biol Lab, Dept Plant Physiol, Tsukuba, Ibaraki 3058602, Japan

来源：

PLANT CELL | 2002年 / 14卷 / 10期

关键词：

D O I：

10.1105/tpc.003061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TAZ1 (TAPETUM DEVELOPMENT ZINC FINGER PROTEIN1; renamed from PEThy; ZPT3-2) cDNA was first isolated as an anther-specific cDNA from petunia. Here, we report a functional characterization that includes analysis of spatial and temporal expression profiles and examination of anther phenotypes in TAZ1-silenced plants. TAZ1 showed a bi-phasic expression pattern. In the premeiotic phase, TAZ1 transcripts were found to accumulate in all cell types of the anther except the tapetum and gametophytic tissues, whereas the postmeiotic phase of anther development was characterized by expression exclusively in the tapetum. Silencing of TAZ1 by cosuppression resulted in aberrant development and precocious degeneration of the tapetum, followed by extensive microspore abortion that started soon after their release from pollen tetrads. A few pollen grains that survived showed reduced flavonol accumulation, defects in pollen wall formation, and poor germination rates. This study demonstrates an essential role for TAZ1 in the postmeiotic phase of tapetum development.

引用

页码：2353 / 2367

页数：15

共 39 条

[11]

KOES RE, 1990, PLANT CELL, V2, P379, DOI 10.1105/tpc.2.5.379

[12] DIFFERENT TEMPORAL AND SPATIAL GENE-EXPRESSION PATTERNS OCCUR DURING ANTHER DEVELOPMENT [J].

KOLTUNOW, AM ;

TRUETTNER, J ;

COX, KH ;

WALLROTH, M ;

GOLDBERG, RB .

PLANT CELL, 1990, 2 (12) :1201-1224

[13] DNA-SYNTHESIS AND CYTOPLASMIC DIFFERENTIATION IN TAPETAL CELLS OF NORMAL AND CYTOPLASMICALLY MALE STERILE LINES OF PETUNIA-HYBRIDA [J].

LIU, XC ;

JONES, K ;

DICKINSON, HG .

THEORETICAL AND APPLIED GENETICS, 1987, 74 (06) :846-851

[14] IMPROVED METHOD FOR THE ISOLATION OF RNA FROM PLANT-TISSUES [J].

LOGEMANN, J ;

SCHELL, J ;

WILLMITZER, L .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (01) :16-20

[15] INDUCTION OF MALE-STERILITY IN PLANTS BY A CHIMERIC RIBONUCLEASE GENE [J].

MARIANI, C ;

DEBEUCKELEER, M ;

TRUETTNER, J ;

LEEMANS, J ;

GOLDBERG, RB .

NATURE, 1990, 347 (6295) :737-741

[16] BIOCHEMICAL COMPLEMENTATION OF CHALCONE SYNTHASE MUTANTS DEFINES A ROLE FOR FLAVONOLS IN FUNCTIONAL POLLEN [J].

MO, YY ;

NAGEL, C ;

TAYLOR, LP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7213-7217

[17] BETA-GLUCURONIDASE ACTIVITY DURING DEVELOPMENT OF THE MALE GAMETOPHYTE FROM TRANSGENIC AND NON-TRANSGENIC PLANTS [J].

PLEGT, L ;

BINO, RJ .

MOLECULAR & GENERAL GENETICS, 1989, 216 (2-3) :321-327

[18]

RAGHAVAN V, 1989, J CELL SCI, V92, P217

[19] A TRANSIENT ACCUMULATION OF POLY(A)-CONTAINING RNA IN THE TAPETUM OF HYOSCYAMUS-NIGER DURING MICROSPOROGENESIS [J].

RAGHAVAN, V .

DEVELOPMENTAL BIOLOGY, 1981, 81 (02) :342-348

[20]

RAGHAVAN V, 1997, MOL EMBRYOLOGY FLOWE, P17

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