HYPERBARIC OXYGEN PRECONDITIONING REDUCES ISCHEMIA-REPERFUSION INJURY BY INHIBITION OF APOPTOSIS VIA MITOCHONDRIAL PATHWAY IN RAT BRAIN

被引：84

作者：

Li, J. -S. ^{[1
]}

Zhang, W. ^{[1
]}

Kang, Z. -M. ^{[2
]}

Ding, S. -J. ^{[1
]}

Liu, W. -W. ^{[2
]}

Zhang, J. H. ^{[3
]}

Guan, Y. -T. ^{[1
]}

Sun, X. -J. ^{[2
]}

机构：

[1] Changhai Hosp, Dept Neurol, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Fac Naval Med, Dept Diving Med, Shanghai 200433, Peoples R China

[3] Loma Linda Univ, Dept Neurosurg, Loma Linda, CA 92350 USA

来源：

NEUROSCIENCE | 2009年 / 159卷 / 04期

关键词：

hyperbaric oxygenation; prevention; middle cerebral artery occlusion; apoptosis; ischemic penumbra; SPINAL-CORD ISCHEMIA; CEREBRAL-ISCHEMIA; TRANSIENT; TOLERANCE; HYPEROXIA; EXPOSURE; INFARCTION; INDUCTION; PENUMBRA; DEATH;

D O I：

10.1016/j.neuroscience.2009.01.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This study examined the hypothesis that apoptotic inhibition via mitochondrial pathway was involved in hyperbaric oxygen preconditioning (HBO-PC)-induced neuroprotection on ischemia-reperfusion injury in rat brain. Male Sprague-Dawley rats (250 similar to 280 g, n=144) were divided into control, middle cerebral artery occlusion (MCAO) for 90 min, and HBO-PC plus MCAO groups. HBO-PC was conducted four times by giving 100% oxygen at 2.5 atm absolute (ATA), for I h at 12 h intervals for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function, brain water content, infarct volume, and cell death were evaluated. Enzymatic activity of capase-3 and -9, and expression of cytochrome c, Bcl-2 and Bax proteins were performed in the samples from hippocampus, ischemic penumbra and core of the brain cortex, respectively. HBO-PC reduced brain edema, decreased infarction volume, and improved neurological recovery. HBO-PC reduced cytoplasm cytochrome c levels, decreased caspase enzyme activity, upregulated the ratio of Bcl-2 and Bax expression, and abated the apoptosis of ischemic tissue. HBO-PC protects brain tissues from ischemia-reperfusion injury by suppressing mitochondrial apoptotic pathways. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：1309 / 1315

页数：7

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