Rapid method for the determination of the substitution pattern of O-methylated 1,4-glucans by high-pH anion-exchange chromatography with pulsed amperometric detection

被引：23

作者：

Heinrich, J ^{[1
]}

Mischnick, P ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ORGAN CHEM,D-20146 HAMBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 749卷 / 1-2期

关键词：

carbohydrates; glucans;

D O I：

10.1016/0021-9673(96)00468-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method has been developed for the determination of the substitution pattern of methyl-starches, -amyloses, -celluloses and -cyclodextrins in the anhydro glucose unit. All eight constituents possible for this type of copolymers could be separated by high-pH anion-exchange chromatography with pulsed amperometric detection (PAD). Peaks were assigned by comparison with synthesized standard compounds. For quantitative evaluation the relative response factors of the O-methyl-glucose derivatives were determined.

引用

收藏

页码：41 / 45

页数：5

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