A strategy for enzyme immobilization on layer-by-layer dendrimer-gold nanoparticle electrocatalytic membrane incorporating redox mediator

被引：148

作者：

Crespilho, Frank N.

Ghica, M. Emilia

Florescu, Monica

Nart, Francisco C.

Oliveira, Osvaldo N., Jr.

Brett, Christopher M. A. ^{[1
]}

机构：

[1] Univ Coimbra, Dept Quim, P-3004535 Coimbra, Portugal

[2] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Paulo, Brazil

[4] Transilvania Univ, Dept Phys, Brasov, Romania

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2006年 / 8卷 / 10期

基金：

巴西圣保罗研究基金会;

关键词：

layer-by-layer; PAMAM dendrimers; gold nanoparticles; enzyme immobilization; redox mediator; biosensor;

D O I：

10.1016/j.elecom.2006.07.032

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A new approach is described to produce nanostructured electrocatalytic membranes using a combination of three methods. Using the layer-by-layer technique, PAMAM dendrimers with cobalt hexacyanoferrates-modified gold nanoparticles were alternated with poly(vinylsulfonic acid) layers on ITO (indium tin oxide) electrodes. This film was used as substrate for immobilization of glucose oxidase in the presence of bovine serum albumin and glutaraldehyde as cross-linker. The modified electrode was successfully applied as a biosensor for the amperometric measurement of glucose, using glucose oxidase enzyme, at 0.0 V vs. SCE. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1665 / 1670

页数：6

共 14 条

[1] Dendrimers as nanoreactors to produce platinum nanoparticles embedded in layer-by-layer films for methanol-tolerant cathodes [J].