Gene sequence and the 1.8 Å crystal structure of the tungsten-containing formate dehydrogenase from Desulfolvibrio gigas

被引:137
作者
Raaijmakers, H
Macieira, S
Dias, JM
Teixeira, S
Bursakov, S
Huber, R
Moura, JJG
Moura, I
Romao, MJ [1 ]
机构
[1] Univ Nova Lisboa, FCT, Dept Quim, REQUIMTE,CQFB, P-2829516 Caparica, Portugal
[2] Max Planck Inst Biochem, D-82152 Martinsried, Germany
关键词
tungsten; selenium; formate dehydrogenase; selenocysteine; molybdopterin; iron-sulfur cluster;
D O I
10.1016/S0969-2126(02)00826-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Desulfiovibrio gigas formate dehydrogenase is the first representative of a tungsten-containing enzyme from a mesophile that has been structurally characterized. It is a heterodimer of 110 and 24 kDa subunits. The large subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase, harbors the W site and one [4Fe-4S] center. No small subunit ortholog containing three [4Fe-4S] clusters has been reported. The structural homology with E. coli FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the active site are conserved. The active site is accessible via a positively charged tunnel, while product release may be facilitated, for H+ by buried waters and protonable amino acids and for CO2 through a hydrophobic channel.
引用
收藏
页码:1261 / 1272
页数:12
相关论文
共 39 条
[21]  
NICHOLLS A, 1991, PROTEIN-STRUCT FUNCT, V11, P134
[22]   Desulfovibrio desulfuricans iron hydrogenase:: the structure shows unusual coordination to an active site Fe binuclear center [J].
Nicolet, Y ;
Piras, C ;
Legrand, P ;
Hatchikian, CE ;
Fontecilla-Camps, JC .
STRUCTURE, 1999, 7 (01) :13-23
[23]   Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites [J].
Nielsen, H ;
Engelbrecht, J ;
Brunak, S ;
vonHeijne, G .
PROTEIN ENGINEERING, 1997, 10 (01) :1-6
[24]  
OCHMAN H, 1988, GENETICS, V120, P621
[25]   Processing of X-ray diffraction data collected in oscillation mode [J].
Otwinowski, Z ;
Minor, W .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326
[26]   Automated protein model building combined with iterative structure refinement [J].
Perrakis, A ;
Morris, R ;
Lamzin, VS .
NATURE STRUCTURAL BIOLOGY, 1999, 6 (05) :458-463
[27]   Tungsten-containing formats dehydrogenase from Desulfovibrio gigas:: metal identification and preliminary structural data by multi-wavelength crystallography [J].
Raaijmakers, H ;
Teixeira, S ;
Dias, JM ;
Almendra, MJ ;
Brondino, CD ;
Moura, I ;
Moura, JJG ;
Romao, MJ .
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2001, 6 (04) :398-404
[28]   Co-translocation of a periplasmic enzyme complex by a hitchhiker mechanism through the bacterial Tat pathway [J].
Rodrigue, A ;
Chanal, A ;
Beck, K ;
Müller, M ;
Wu, LF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (19) :13223-13228
[29]  
Romao MJ, 1997, PROG BIOPHYS MOL BIO, V68, P121, DOI 10.1016/S0079-6107(97)00022-9
[30]  
ROMAO MJ, 1995, SCIENCE, V270, P1170, DOI 10.1126/science.270.5239.1170