Gene expression profiling of cell lines derived from T-cell malignancies

被引:13
作者
Fillmore, GC
Lin, ZS
Bohling, SD
Robetorye, RS
Kim, CH
Jenson, SD
Elenitoba-Johnson, KSJ
Lim, MS
机构
[1] Univ Utah, Hlth Sci Ctr, Dept Pathol, Salt Lake City, UT 84132 USA
[2] Univ Utah, Hlth Sci Ctr, ARUP Inst Clin & Expt Pathol, Salt Lake City, UT 84132 USA
基金
美国国家卫生研究院;
关键词
T-cell; microarray; expression profiling;
D O I
10.1016/S0014-5793(02)02914-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The expression profiles of eight cell lines derived from T-cell malignancies were compared to CD4-positive T-cells using cDNA microarray technology. Unsupervised hierarchical clustering of 4364 genes demonstrated substantial heterogeneity resulting in four distinct groups. While no genes were found to be uniformly up- or down-regulated across all cell lines, we observed 111 over-expressed genes (greater than two-fold) and 1118 down-regulated genes (greater than two-fold) in the lymphomas as a group when compared to CD4-positive T-cells. These included genes involved in cytokine signaling, cell adhesion, cytoskeletal elements, nuclear transcription factors, and known oncogenes and tumor suppressor genes. Quantitative fluorescent reverse transcription-polymerase chain reaction analysis demonstrated 70% concordance with the microarray results. While freshly isolated malignant cells may differ in their individual expression patterns relative to established cell lines from the same diagnoses, we feel that the variety of different lymphocytic cell lines that we examined provides a representative picture of the molecular pathogenesis of T-cell malignancies. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:183 / 188
页数:6
相关论文
共 44 条
[1]   The lymphochip: A specialized cDNA microarray for the genomic-scale analysis of gene expression in normal and malignant lymphocytes [J].
Alizadeh, A ;
Eisen, M ;
Davis, RE ;
Ma, C ;
Sabet, H ;
Tran, T ;
Powell, JI ;
Yang, L ;
Marti, GE ;
Moore, DT ;
Hudson, JR ;
Chan, WC ;
Greiner, T ;
Weisenburger, D ;
Armitage, JO ;
Lossos, I ;
Levy, R ;
Botstein, D ;
Brown, PO ;
Staudt, LM .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1999, 64 :71-78
[2]   Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling [J].
Alizadeh, AA ;
Eisen, MB ;
Davis, RE ;
Ma, C ;
Lossos, IS ;
Rosenwald, A ;
Boldrick, JG ;
Sabet, H ;
Tran, T ;
Yu, X ;
Powell, JI ;
Yang, LM ;
Marti, GE ;
Moore, T ;
Hudson, J ;
Lu, LS ;
Lewis, DB ;
Tibshirani, R ;
Sherlock, G ;
Chan, WC ;
Greiner, TC ;
Weisenburger, DD ;
Armitage, JO ;
Warnke, R ;
Levy, R ;
Wilson, W ;
Grever, MR ;
Byrd, JC ;
Botstein, D ;
Brown, PO ;
Staudt, LM .
NATURE, 2000, 403 (6769) :503-511
[3]   New approach to classifying non-hodgkin's lymphomas: Clinical features of the major histologic subtypes [J].
Armitage, JO ;
Weisenburger, DD .
JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (08) :2780-2795
[4]   T-CELL RECEPTOR GENE REARRANGEMENTS AND THE DIAGNOSIS OF HUMAN T-CELL NEOPLASMS [J].
COSSMAN, J ;
UPPENKAMP, M ;
ANDRADE, R ;
MEDEIROS, LJ .
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 1990, 10 (03) :267-281
[5]   HODGKINS-DISEASE, LYMPHOMATOID PAPULOSIS, AND CUTANEOUS T-CELL LYMPHOMA DERIVED FROM A COMMON T-CELL CLONE [J].
DAVIS, TH ;
MORTON, CC ;
MILLERCASSMAN, R ;
BALK, SP ;
KADIN, ME .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (17) :1115-1122
[6]  
DeRisi J, 1996, NAT GENET, V14, P457
[7]   Cluster analysis and display of genome-wide expression patterns [J].
Eisen, MB ;
Spellman, PT ;
Brown, PO ;
Botstein, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868
[8]  
EPSTEIN AL, 1974, CANCER-AM CANCER SOC, V34, P1851, DOI 10.1002/1097-0142(197412)34:6<1851::AID-CNCR2820340602>3.0.CO
[9]  
2-4
[10]   DEREGULATION OF C-MYC BY TRANSLOCATION OF THE ALPHA-LOCUS OF THE T-CELL RECEPTOR IN T-CELL LEUKEMIAS [J].
ERIKSON, J ;
FINGER, L ;
SUN, L ;
ARRUSHDI, A ;
NISHIKURA, K ;
MINOWADA, J ;
FINAN, J ;
EMANUEL, BS ;
NOWELL, PC ;
CROCE, CM .
SCIENCE, 1986, 232 (4752) :884-886