Simple method for plant virus detection: effect of antibody immobilization technique

被引：21

作者：

Boltovets, PM

Boyko, VR

Kostikov, IY

Dyachenko, NS

Snopok, BA

Shirshov, YM

机构：

[1] Natl Acad Sci Ukraine, DK Zabolotny Inst Microbiol & Virol, UA-03143 Kiev, Ukraine

[2] Taras Shevchenko Natl Univ, Kiev, Ukraine

[3] Natl Acad Sci, Inst Semicond Phys, UA-03028 Kiev, Ukraine

来源：

JOURNAL OF VIROLOGICAL METHODS | 2002年 / 105卷 / 01期

关键词：

oriented protein immobilization; surface plasmon resonance; TMV;

D O I：

10.1016/S0166-0934(02)00098-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment. namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A (Staphylococcus aurens), the latter was chosen as most suited for retention of the formed native immunoglobulin layer. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：141 / 146

页数：6

共 25 条

[1] DETERMINATION OF KINETIC CONSTANTS FOR THE INTERACTION BETWEEN A MONOCLONAL-ANTIBODY AND PEPTIDES USING SURFACE-PLASMON RESONANCE [J].