Prediction of heat-induced collagen shrinkage by use of second harmonic generation microscopy

被引:47
作者
Lin, Sung-Jan
Lo, Wen
Tan, Hsin-Yuan
Chan, Jung-Yi
Chen, Wei-Liang
Wang, Shiou-Han
Sun, Yen
Lin, Wei-Chou
Chen, Jau-Shiuh
Hsu, Chih-Jung
Tjiu, Jeng-Wei
Yu, Hsin-Su
Jee, Shiou-Hwa [1 ]
Dong, Chen-Yuan
机构
[1] Natl Taiwan Univ Hosp, Dept Dermatol, Taipei 100, Taiwan
[2] Coll Med, Dept Dermatol, Taipei, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Dermatol, Yunlin Branch, Touliu, Yunlin, Taiwan
[4] Natl Taiwan Univ, Dept Phys, Taipei, Taiwan
[5] Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
[6] Natl Taiwan Univ, Inst Biomed Engn, Taipei, Taiwan
[7] Cathay Gen Hosp, Dept Dermatol, Taipei, Taiwan
[8] Natl Taiwan Univ Hosp, Dept Pathol, Taipei 100, Taiwan
关键词
second harmonic generation; collagen; denaturation; shrinkage; thermal;
D O I
10.1117/1.2209959
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Collagen shrinkage associated with denaturation from thermal treatment has a number of important clinical applications. However, individualized treatment is hindered by the lack of reliable noninvasive methods to monitor the process of collagen denaturation. We investigate the serial changes of collagen denaturation from thermal treatment of rat tail tendons at 58 degrees C by use of second harmonic generation (SHG) microscopy. We find that rat tail tendon shrinks progressively from 0 to 9 min of thermal treatment, and remains unchanged in length upon further thermal treatment. The SHG intensity also decreases from 0 to 9 min of thermal treatment and becomes barely detectable from further thermal treatment. Collagen shrinkage and the SHG intensity are well correlated in a linear model. In addition, SHG imaging reveals a tiger-tail-like pattern of collagen denaturation. The bands of denatured collagen progressively widen from increased thermal treatment and completely replace the adjacent bands of normal collagen after 9 min of thermal treatment. Our results show that collagen denaturation in rat tail tendon from thermal treatment is inhomogeneous, and that SHG intensity can be used to predict the degree of thermally induced collagen shrinkage. With additional development, this approach has the potential to be used in biomedical applications. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
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页数:6
相关论文
共 46 条
[1]   Improvement of neck and cheek laxity with a nonablative radiofrequency device: A lifting experience [J].
Alster, TS ;
Tanzi, E .
DERMATOLOGIC SURGERY, 2004, 30 (04) :503-507
[2]   Treatment of facial rhytides with a high-energy pulsed carbon dioxide laser [J].
Alster, TS ;
Garg, S .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1996, 98 (05) :791-794
[3]   THE NATIVE AND DENATURED STATES OF SOLUBLE COLLAGEN [J].
BOEDTKER, H ;
DOTY, P .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1956, 78 (17) :4267-4280
[4]   Different pattern of collagen cross-links in two sclerotic skin diseases:: Lipodermatosclerosis and circumscribed scleroderma [J].
Brinckmann, J ;
Neess, CM ;
Gaber, Y ;
Sobhi, H ;
Notbohm, H ;
Hunzelmann, N ;
Fietzek, PP ;
Müller, PK ;
Risteli, J ;
Gebker, R ;
Scharffetter-Kochanek, K .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (02) :269-273
[5]   Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation [J].
Brown, E ;
McKee, T ;
diTomaso, E ;
Pluen, A ;
Seed, B ;
Boucher, Y ;
Jain, RK .
NATURE MEDICINE, 2003, 9 (06) :796-800
[6]   RELATIONSHIP BETWEEN THE CONTENT OF LYSYL OXIDASE DEPENDENT CROSS-LINKS IN SKIN COLLAGEN, NONENZYMATIC GLYCOSYLATION, AND LONG-TERM COMPLICATIONS IN TYPE-I DIABETES-MELLITUS [J].
BUCKINGHAM, B ;
REISER, KM .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (04) :1046-1054
[7]   THERMAL DENATURATION OF COLLAGEN IN SOLUTION AND ITS STRUCTURAL IMPLICATIONS [J].
BURGE, RE ;
HYNES, RD .
JOURNAL OF MOLECULAR BIOLOGY, 1959, 1 (02) :155-164
[8]   Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms [J].
Campagnola, PJ ;
Loew, LM .
NATURE BIOTECHNOLOGY, 2003, 21 (11) :1356-1360
[9]  
CHEN SS, 1998, ASME, V120, P382
[10]   Ultrahigh-resolution ophthalmic optical coherence tomography [J].
Drexler, W ;
Morgner, U ;
Ghanta, RK ;
Kärtner, FX ;
Schuman, JS ;
Fujimoto, JG .
NATURE MEDICINE, 2001, 7 (04) :502-507