Prediction of heat-induced collagen shrinkage by use of second harmonic generation microscopy

被引:47
作者
Lin, Sung-Jan
Lo, Wen
Tan, Hsin-Yuan
Chan, Jung-Yi
Chen, Wei-Liang
Wang, Shiou-Han
Sun, Yen
Lin, Wei-Chou
Chen, Jau-Shiuh
Hsu, Chih-Jung
Tjiu, Jeng-Wei
Yu, Hsin-Su
Jee, Shiou-Hwa [1 ]
Dong, Chen-Yuan
机构
[1] Natl Taiwan Univ Hosp, Dept Dermatol, Taipei 100, Taiwan
[2] Coll Med, Dept Dermatol, Taipei, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Dermatol, Yunlin Branch, Touliu, Yunlin, Taiwan
[4] Natl Taiwan Univ, Dept Phys, Taipei, Taiwan
[5] Chang Gung Mem Hosp, Dept Ophthalmol, Linkou, Taiwan
[6] Natl Taiwan Univ, Inst Biomed Engn, Taipei, Taiwan
[7] Cathay Gen Hosp, Dept Dermatol, Taipei, Taiwan
[8] Natl Taiwan Univ Hosp, Dept Pathol, Taipei 100, Taiwan
关键词
second harmonic generation; collagen; denaturation; shrinkage; thermal;
D O I
10.1117/1.2209959
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Collagen shrinkage associated with denaturation from thermal treatment has a number of important clinical applications. However, individualized treatment is hindered by the lack of reliable noninvasive methods to monitor the process of collagen denaturation. We investigate the serial changes of collagen denaturation from thermal treatment of rat tail tendons at 58 degrees C by use of second harmonic generation (SHG) microscopy. We find that rat tail tendon shrinks progressively from 0 to 9 min of thermal treatment, and remains unchanged in length upon further thermal treatment. The SHG intensity also decreases from 0 to 9 min of thermal treatment and becomes barely detectable from further thermal treatment. Collagen shrinkage and the SHG intensity are well correlated in a linear model. In addition, SHG imaging reveals a tiger-tail-like pattern of collagen denaturation. The bands of denatured collagen progressively widen from increased thermal treatment and completely replace the adjacent bands of normal collagen after 9 min of thermal treatment. Our results show that collagen denaturation in rat tail tendon from thermal treatment is inhomogeneous, and that SHG intensity can be used to predict the degree of thermally induced collagen shrinkage. With additional development, this approach has the potential to be used in biomedical applications. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
引用
收藏
页数:6
相关论文
共 46 条
[41]   Denaturation of collagen via heating: An irreversible rate process [J].
Wright, NT ;
Humphrey, JD .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :109-128
[42]  
YAMAUCHI M, 1991, J INVEST DERMATOL, V97, P938
[43]   Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry [J].
Yasui, T ;
Tohno, Y ;
Araki, T .
JOURNAL OF BIOMEDICAL OPTICS, 2004, 9 (02) :259-264
[44]   Reversible dissociation of collagen in tissues [J].
Yeh, AT ;
Choi, B ;
Nelson, JS ;
Tromberg, BJ .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2003, 121 (06) :1332-1335
[45]   Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation [J].
Zipfel, WR ;
Williams, RM ;
Christie, R ;
Nikitin, AY ;
Hyman, BT ;
Webb, WW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) :7075-7080
[46]   Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence [J].
Zoumi, A ;
Yeh, A ;
Tromberg, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :11014-11019