Establishment of a host-vector system in Bacillus megaterium strain NK84-0128, an oxetanocin A producer

被引：1

作者：

Morita, M

Tomita, K

Sato, S

Morino, T

机构：

[1] Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co, Tokyo, 115, 3–31–12 Shimo, Kita-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 06期

关键词：

host-vector system; Bacillus megaterium; oxetanocin A; protoplast transformation; chloramphenicol acetyltransferase gene;

D O I：

10.1271/bbb.60.1020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A host-vector system was constructed in Bacillus megaterium strain NK84-0128, an oxetanocin A producer. The replication origin of an endogeneous plasmid, P-4, was used to construct a potential plasmid vector, pSM5, which had a chloramphenicol resistance gene as a selective marker. Plasmid transformation by a protoplast method was used in B. megaterium strain NK84-0128. The maximum transformation frequency attained with the pSM5 plasmid was 2.0 x 10(4) cfu/mu g DNA.

引用

页码：1020 / 1022

页数：3

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