Cytosine-5-methyltransferases add aldehydes to DNA

被引：108

作者：

Liutkeviciute, Zita ^{[1
]}

Lukinavicius, Grazvydas ^{[1
]}

Masevicius, Viktoras ^{[1
,2
]}

Daujotyte, Dalia ^{[1
]}

Klimasauskas, Saulius ^{[1
]}

机构：

[1] Inst Biotechnol, Lab Biol DNA Modificat, Vilnius, Lithuania

[2] Vilnius State Univ, Dept Chem, Vilnius, Lithuania

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 06期

关键词：

METHYLTRANSFERASE; FORMALDEHYDE; 5-METHYLCYTOSINE; DEMETHYLATION; METHYLATION; DEAMINATION; MECHANISM; ENZYMES; HELIX; BASE;

D O I：

10.1038/nchembio.172

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Targeted methylation of cytosine residues by S-adenosylmethionine-dependent DNA methyltransferases modulates gene expression in vertebrates. Here we show that cytosine-5-methyltransferases catalyze reversible covalent addition of exogenous aliphatic aldehydes to their target residues in DNA, thus yielding corresponding 5-alpha-hydroxyalkylcytosines. Such atypical enzymatic reactions with non-cofactor-like substrates open new ways for sequence-specific derivatization of DNA and demonstrate enzymatic exchange of 5-hydroxymethyl groups on cytosine in support of an oxidative mechanism of DNA demethylation.

引用

页码：400 / 402

页数：3

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