Purification of icariin from the extract of Epimedium segittatum using high-speed counter-current chromatography

被引:35
作者
Du, QZ
Xia, M
Ito, Y
机构
[1] NHLBI, Lab Biophys Chem, NIH, Bethesda, MD 20892 USA
[2] Hangzhou Univ Commerce, Inst Food & Biol Engn, Hangzhou 310035, Peoples R China
关键词
Epimedium segittatum; counter-current chromatography; icariin;
D O I
10.1016/S0021-9673(02)00538-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Icariin was purified from the extract of Epimedium segittatum by high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-n-butanol-methanol-water (1:4:2:6, v/v). We used two multilayer coil planet centrifuges with different capacities. A 300 mg amount of the extract was separated using a semipreparative instrument equipped with a 230-ml capacity column to yield 103 mg of icariin at 86.2% purity. An 8 g amount of the extract was separated with a large preparative instrument equipped with a 2460-ml capacity column to produce 2.45 g of icariin at 85.7% purity. From these fractions over 98% pure icariin was obtained by recrystallization with water. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:239 / 241
页数:3
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