Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens

被引：26

作者：

Fogg, M. J.

Alzari, P.

Bahar, M.

Bertini, I.

Betton, J. -M.

Burmeister, W. P.

Cambillau, C.

Canard, B.

Carrondo, M.

Coll, M.

Daenke, S.

Dym, O.

Egloff, M. -P.

Enguita, F. J.

Geerlof, A.

Haouz, A.

Jones, T. A.

Ma, Qingjun

Manicka, S. N.

Migliardi, M.

Nordlund, P.

Owens, R. J.

Peleg, Y.

Schneider, G.

Schnell, R.

Stuart, D. I.

Tarbouriech, N.

Unge, T.

Wilkinson, A. J.

Wilmanns, M.

Wilson, K. S.

Zimhony, O.

Grimes, J. M.

机构：

[1] Wellcome Trust Ctr Human Genet, Div Struct Biol, Oxford OX3 7BN, England

[2] Univ York, York Struct Biol Lab, Dept Chem, York YO10 5YW, N Yorkshire, England

[3] Inst Pasteur, Unite Biochim Struct, F-75724 Paris 15, France

[4] CERM, CIRMMP, I-50019 Sesto Fiorentino, Italy

[5] EMBL Grenoble, ILL, F-38042 Grenoble 9, France

[6] Univ Aix Marseille 1, CNRS, Architecture & Fonct Macromol Biol UMR 6098, F-13288 Marseille 09, France

[7] Inst Tecnol Quim & Biol, Macromol Crystallog Lab, Host Pathogen Interact Grp, P-2781901 Oeiras, Portugal

[8] Inst Mol Biol, Dept Biol Estructural, Barcelona, Spain

[9] Weizmann Inst Sci, Israel Struct Proteom Ctr, Dept Biol Struct, IL-76100 Rehovot, Israel

[10] EMBL, Hamburg Outstn, D-22603 Hamburg, Germany

[11] Uppsala Univ, Dept Biol Mol, Ctr Biomed, S-75124 Uppsala, Sweden

[12] Univ Stockholm, Dept Biochem & Biophys, S-10691 Stockholm, Sweden

[13] Karolinska Inst, Dept Med Biochem & Biophys, SE-10951 Stockholm, Sweden

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷

基金：

英国医学研究理事会;

关键词：

D O I：

10.1107/S0907444906030915

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Structural Proteomics In Europe ( SPINE) programme is aimed at the development and implementation of highthroughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (similar to 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.

引用

页码：1196 / 1207

页数：12

共 63 条

[1] NMR in the SPINE structural proteomics project [J].

Ab, E. ;

Atkinson, A. R. ;

Banci, L. ;

Bertini, I. ;

Ciofi-Baffoni, S. ;

Brunner, K. ;

Diercks, T. ;

Doetsch, V. ;

Engelke, F. ;

Folkers, G. E. ;

Griesinger, C. ;

Gronwald, W. ;

Guenther, U. ;

Habeck, M. ;

de Jong, R. N. ;

Kalbitzer, H. R. ;

Kieffer, B. ;

Leeflang, B. R. ;

Loss, S. ;

Luchinat, C. ;

Marquardsen, T. ;

Moskau, D. ;

Neidig, K. -P. ;

Nilges, M. ;

Piccioli, M. ;

Pierattelli, R. ;

Rieping, W. ;

Schippmann, T. ;

Schwalbe, H. ;

Trave, G. ;

Trenner, J. ;

Woehnert, J. ;

Zweckstetter, M. ;

Kaptein, R. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1150-1161

[2] SPINE bioinformatics and data-management aspects of high-throughput structural biology [J].

Albeck, S. ;

Alzari, P. ;

Andreini, C. ;

Banci, L. ;

Berry, I. M. ;

Bertini, I. ;

Cambillau, C. ;

Canard, B. ;

Carter, L. ;

Cohen, S. X. ;

Diprose, J. M. ;

Dym, O. ;

Esnouf, R. M. ;

Felder, C. ;

Ferron, F. ;

Guillemot, F. ;

Hamer, R. ;

Ben Jelloul, M. ;

Laskowski, R. A. ;

Laurent, T. ;

Longhi, S. ;

Lopez, R. ;

Luchinat, C. ;

Malet, H. ;

Mochel, T. ;

Morris, R. J. ;

Moulinier, L. ;

Oinn, T. ;

Pajon, A. ;

Peleg, Y. ;

Perrakis, A. ;

Poch, O. ;

Prilusky, J. ;

Rachedi, A. ;

Ripp, R. ;

Rosato, A. ;

Silman, I. ;

Stuart, D. I. ;

Sussman, J. L. ;

Thierry, J. -C. ;

Thompson, J. D. ;

Thornton, J. M. ;

Unger, T. ;

Vaughan, B. ;

Vranken, W. ;

Watson, J. D. ;

Whamond, G. ;

Henrick, K. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1184-1195

[3] Vaccinia virus strains Lister, USSR and Evans express soluble and cell-surface tumour necrosis factor receptors [J].

Alcamí, A ;

Khanna, A ;

Paul, NL ;

Smith, GL .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :949-959

[4] Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon [J].

Alekshun, MN ;

Levy, SB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (10) :2067-2075

[5] Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE [J].

Alzari, Pedro M. ;

Berglund, H. ;

Berrow, N. S. ;

Blagova, E. ;

Busso, D. ;

Cambillau, C. ;

Campanacci, V. ;

Christodoulou, E. ;

Eiler, S. ;

Fogg, M. J. ;

Folkers, G. ;

Geerlof, A. ;

Hart, D. ;

Haouz, A. ;

Herman, M. D. ;

Macieira, S. ;

Nordlund, P. ;

Perrakis, A. ;

Quevillon-Cheruel, S. ;

Tarandeau, F. ;

van Tilbeurgh, H. ;

Unger, T. ;

Luna-Vargas, M. P. A. ;

Velarde, M. ;

Willmanns, M. ;

Owens, Raymond J. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1103-1113

[6] Eukaryotic expression: developments for structural proteomics [J].

Aricescu, A. R. ;

Assenberg, R. ;

Bill, R. M. ;

Busso, D. ;

Chang, V. T. ;

Davis, S. J. ;

Dubrovsky, A. ;

Gustafsson, L. ;

Hedfalk, K. ;

Heinemann, U. ;

Jones, I. M. ;

Ksiazek, D. ;

Lang, C. ;

Maskos, K. ;

Messerschmidt, A. ;

Macieira, S. ;

Peleg, Y. ;

Perrakis, A. ;

Poterszman, A. ;

Schneider, G. ;

Sixma, T. K. ;

Sussman, J. L. ;

Sutton, G. ;

Tarboureich, N. ;

Zeev-Ben-Mordehai, T. ;

Jones, E. Yvonne .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1114-1124

[7] A time- and cost-efficient system for high-level protein production in mammalian cells [J].

Aricescu, A. Radu ;

Lu, Weixian ;

Jones, E. Yvonne .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :1243-1250

[8] Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis [J].

Au, K. ;

Berrow, N. S. ;

Blagova, E. ;

Boucher, I. W. ;

Boyle, M. P. ;

Brannigan, J. A. ;

Carter, L. G. ;

Dierks, T. ;

Folkers, G. ;

Grenha, R. ;

Harlos, K. ;

Kaptein, R. ;

Kalliomaa, A. K. ;

Levdikov, V. M. ;

Meier, C. ;

Milioti, N. ;

Moroz, O. ;

Muller, A. ;

Owens, R. J. ;

Rzechorzek, N. ;

Sainsbury, S. ;

Stuart, D. I. ;

Walter, T. S. ;

Waterman, D. G. ;

Wilkinson, A. J. ;

Wilson, K. S. ;

Zaccai, N. ;

Esnouf, Robert M. ;

Fogg, Mark J. .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2006, 62 :1267-1275

[9] First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value [J].

Banci, L. ;

Bertini, I. ;

Cusack, S. ;

de Jong, R. N. ;

Heinemann, U. ;

Jones, E. Y. ;

Kozielski, F. ;

Maskos, K. ;

Messerschmidt, A. ;

Owens, R. ;

Perrakis, A. ;

Poterszman, A. ;

Schneider, G. ;

Siebold, C. ;

Silman, I. ;

Sixma, T. ;

Stewart-Jones, G. ;

Sussman, J. L. ;

Thierry, J. -C. ;

Moras, Dino .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2006, 62 :1208-1217

[10] Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis [J].

Banci, L ;

Bertini, I ;

Ciofi-Baffoni, S ;

Gonnelli, L ;

Su, XC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (50) :50506-50513

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