Recycling of intact dense core vesicles in neurites of NGF-treated PC12 cells

被引:14
作者
Bauer, RA [1 ]
Khera, RS [1 ]
Lieber, JL [1 ]
Angleson, JK [1 ]
机构
[1] Univ Denver, Dept Sci Biol, Denver, CO 80208 USA
来源
FEBS LETTERS | 2004年 / 571卷 / 1-3期
关键词
endocytosis; exocytosis; chromogranin; calcineurin;
D O I
10.1016/j.febslet.2004.05.086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Exocytic fusion in neuroendocrine cells does not always result in complete release of the peptide contents from dense core vesicles (DCVs). In this study, we use fluorescence imaging and immunoelectron microscopy to examine the retention, endocytosis and recycling of chromogranin B in DCVs of NGF-treated PC12 cells. Our results indicate that DCVs retained and retrieved an intact core that was available for subsequent exocytic release. The endocytic process was inhibited by cyclosporine A or by substitution of extracellular Ca2+ with Ba2+, and the total recycling time was less than 5 min. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:107 / 111
页数:5
相关论文
共 34 条
  • [1] The exocytotic event in chromaffin cells revealed by patch amperometry
    Albillos, A
    Dernick, G
    Horstmann, H
    Almers, W
    deToledo, GA
    Lindau, M
    [J]. NATURE, 1997, 389 (6650) : 509 - 512
  • [2] Regulation of dense cove release from neuroendocrine cells revealed by imaging single exocytic events
    Angleson, JK
    Cochilla, AJ
    Kilic, G
    Nussinovitch, I
    Betz, WJ
    [J]. NATURE NEUROSCIENCE, 1999, 2 (05) : 440 - 446
  • [3] Secretion: Dense-core vesicles can kiss-and-run too
    Artalejo, CR
    Elhamdani, A
    Palfrey, HC
    [J]. CURRENT BIOLOGY, 1998, 8 (02) : R62 - R65
  • [4] Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells
    Artalejo, CR
    Elhamdani, A
    Palfrey, HC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (09) : 6358 - 6363
  • [5] RAPID ENDOCYTOSIS COUPLED TO EXOCYTOSIS IN ADRENAL CHROMAFFIN CELLS INVOLVES CA2+, GTP, AND DYNAMIN BUT NOT CLATHRIN
    ARTALEJO, CR
    HENLEY, JR
    MCNIVEN, MA
    PALFREY, CH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) : 8328 - 8332
  • [6] Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells
    Barg, S
    Olofsson, CS
    Schriever-Abeln, J
    Wendt, A
    Gebre-Medhin, S
    Renström, E
    Rorsman, P
    [J]. NEURON, 2002, 33 (02) : 287 - 299
  • [7] Retention and stimulus-dependent recycling of dense core vesicle content in neuroendocrine cells
    Bauer, RA
    Overlease, RL
    Lieber, JL
    Angleson, JK
    [J]. JOURNAL OF CELL SCIENCE, 2004, 117 (11) : 2193 - 2202
  • [8] The synaptic vesicle cycle
    Betz, WJ
    Angleson, JK
    [J]. ANNUAL REVIEW OF PHYSIOLOGY, 1998, 60 : 347 - 363
  • [9] Two forms of triggered endocytosis in regulated secretory cells
    Burgoyne, RD
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1998, 506 (03): : 589 - 589
  • [10] Low frequency stimulation of mouse adrenal slices reveals a clathrin-independent, protein kinase C-mediated endocytic mechanism
    Chan, SA
    Smith, C
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2003, 553 (03): : 707 - 717