Genetically modified organisms in food-screening and specific detection by polymerase chain reaction

被引：114

作者：

Vollenhofer, S ^{[1
]}

Burg, K ^{[1
]}

Schmidt, J ^{[1
]}

Kroath, H ^{[1
]}

机构：

[1] Austraian Res Ctr Siebersdorf, Biotechnol Unit, A-2444 Seibersdorf, Austria

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 12期

关键词：

Polymerase Chain Reaction; PCR; genetically modified organisms; food analysis;

D O I：

10.1021/jf990353l

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

PCR methods for the detection of genetically modified organisms (GMOs) were developed that can be used for screening purposes and for specific detection of glyphosate-tolerant soybean and insect-resistant maize in food. Primers were designed to amplify parts of the 35S promoter derived from Cauliflower Mosaic Virus, the NOS terminator derived from Agrobacterium tunefaciens and the antibiotic marker gene NPTII (neomycin-phosphotransferase II), to allow for general screening of foods. PCR/hybridization protocols were established for the detection of glyphosate-tolerant RoundUp Ready soybean and insect-resistant Bt-maize. Besides hybridization, confirmation of the results using restriction analysis was also possible. The described methods enabled a highly sensitive and specific detection of GMOs and thus provide a useful tool for routine analysis of raw and processed food products.

引用

页码：5038 / 5043

页数：6

共 17 条

[1] POLYMERASE CHAIN-REACTION (PCR) - A POSSIBLE ALTERNATIVE TO IMMUNOCHEMICAL METHODS ASSURING SAFETY AND QUALITY OF FOOD - DETECTION OF WHEAT CONTAMINATION IN NON-WHEAT FOOD-PRODUCTS
ALLMANN, M
CANDRIAN, U
HOFELEIN, C
LUTHY, J
[J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1993, 196 (03): : 248 - 251
[2] GASSER CS, 1988, J BIOL CHEM, V263, P4280
[3] Genome size and maturity group in Glycine max (soybean)
Greilhuber J.
Obermayer R.
[J]. Heredity, 1997, 78 (5) : 547 - 551
[4] Detection of the starch modifying gbss-antisense construct in transgenic potatoes
Hassan-Hauser, C
Mayer, W
Hortner, H
[J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1998, 206 (02): : 83 - 87
[5] Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction
Hupfer, C
Hotzel, H
Sachse, K
Engel, KH
[J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1998, 206 (03): : 203 - 207
[6] FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS-THURINGIENSIS
KOZIEL, MG
BELAND, GL
BOWMAN, C
CAROZZI, NB
CRENSHAW, R
CROSSLAND, L
DAWSON, J
DESAI, N
HILL, M
KADWELL, S
LAUNIS, K
LEWIS, K
MADDOX, D
MCPHERSON, K
MEGHJI, MR
MERLIN, E
RHODES, R
WARREN, GW
WRIGHT, M
EVOLA, SV
[J]. BIO-TECHNOLOGY, 1993, 11 (02): : 194 - 200
[7] Detection of genetically engineered plants by polymerase chain reaction (PCR) using the FLAVR SAVR(TM) tomato as an example
Meyer, R
[J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1995, 201 (06): : 583 - 586
[8] Polymerase chain reaction (PCR) in the quality and safety assurance of food: Detection of soya in processed meat products
Meyer, R
Chardonnens, F
Hubner, P
Luthy, J
[J]. ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1996, 203 (04): : 339 - 344
[9] COMPARISON OF PLANT DNA CONTENTS DETERMINED BY FEULGEN MICROSPECTROPHOTOMETRY AND LASER FLOW-CYTOMETRY
MICHAELSON, MJ
PRICE, HJ
ELLISON, JR
JOHNSTON, JS
[J]. AMERICAN JOURNAL OF BOTANY, 1991, 78 (02) : 183 - 188
[10] DEVELOPMENT, IDENTIFICATION, AND CHARACTERIZATION OF A GLYPHOSATE-TOLERANT SOYBEAN LINE
PADGETTE, SR
KOLACZ, KH
DELANNAY, X
RE, DB
LAVALLEE, BJ
TINIUS, CN
RHODES, WK
OTERO, YI
BARRY, GF
EICHHOLTZ, DA
PESCHKE, VM
NIDA, DL
TAYLOR, NB
KISHORE, GM
[J]. CROP SCIENCE, 1995, 35 (05) : 1451 - 1461

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