Quantification of hemorphin-7 peptides by enzyme linked immunosorbent assay with secondary antibody

被引：7

作者：

Cohen, M

Fruitier-Arnaudin, I

Garreau-Balandier, I

Piot, JM

机构：

[1] Univ Rochelle, Lab Genie Prote & Cellulaire, F-17042 La Rochelle, France

[2] Univ Clermont Ferrand, ERTAC, F-63177 Clermont Ferrand, France

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 461卷 / 02期

关键词：

hemorphin-7; peptides; ELISA;

D O I：

10.1016/S0003-2670(02)00282-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

This paper describes an enzyme linked immunosorbent assay (ELISA) for the quantification of bioactive peptides: hemorphin-7 peptides (LVV-hemorphin-7, VV-hemorphin-7 and hemorphin-7) using an anti-rabbit secondary antibody conjugated to peroxidase for sandwich antibody assay. Bovine serum albumin (BSA) conjugated to VV-hemorphin-7 was readily coated on a polystyrene microplate. Data acquisition on microtiter wells is performed by an ELISA reader. The standard curve was produced for 1 x 10(-13) to 2 x 10(-5) M VV-hemorphin-7. The minimum detectable amount of VV-hemorphin-7 is 5 x 10(-13) mol and the relative standard deviation was 8.8% for a 1 x 10(-6) M sample (n = 8). The ELISA procedure was selective with respect to structurally similar compounds. This method was applied to quantify hemorphin-7 peptides in bovine plasma. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：229 / 233

页数：5

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