Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods

被引：15

作者：

Bhosale, Prakash ^{[1
]}

Serban, Bogdan ^{[1
]}

Bernstein, Paul S. ^{[1
]}

机构：

[1] Univ Utah, Sch Med, Moran Eye Ctr, Dept Ophthalmol & Visual Sci, Salt Lake City, UT 84132 USA

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 17期

关键词：

deuterated lutein; Chlorella protothecoides; HPLC/atmospheric pressure chemical ionization-mass spectrometry; lutein;

D O I：

10.1007/s10529-006-9105-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed 58% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 599. The rapidly growing microalga had much higher levels (58%) of deuterium substitution relative to previously reported (9-15%) natural sources of lutein.

引用

页码：1371 / 1375

页数：5

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