Nuclear DNA fragmentation during postnatal tooth development of mouse and hamster and during dentin repair in the rat

被引:54
作者
Bronckers, ALJJ
Lyaruu, DM
Goei, W
Litz, M
Luo, G
Karsenty, G
Woltgens, JHM
DSouza, RN
机构
[1] FREE UNIV AMSTERDAM,DEPT ORAL CELL BIOL ACTA,AMSTERDAM,NETHERLANDS
[2] UNIV TEXAS,MED CTR,DENT BRANCH,DEPT ANAT SCI,HOUSTON,TX
[3] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,HOUSTON,TX 77030
关键词
amelogenesis; apoptosis; dentin repair; in situ;
D O I
10.1111/j.1600-0722.1996.tb00053.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The TUNEL (transferase-mediated, dUTP-biotin nick end labeling) method for in situ labeling of DNA strands was utilized to localize DNA fragmentation in cells involved in tooth formation In the neonatal mouse and hamster. Positive reactions for the presence of DNA fragments were obtained in some epithelial cells of the cervical loop region of incisors, late secretory, transitional and early maturation stage ameloblasts, stratum intermedium cells and in shortened ameloblasts just before eruption. Also, cells of the periodontal Ligament of the continuously erupting incisors stained positive shortly before eruption, Odontoblasts were negative but became strongly positive during the formation of physiological osteodentin at the tip of developing incisors. Osteodentin matrix and the surfaces of unerupted enamel and cementum just prior to eruption stained for DNA fragments as well. DNA fragmentation could be elicited in odontoblasts and underlying pulpal tissues of mature erupted molars after mechanical injury to the odontoblast processes during cavity preparation. We conclude that, in rodents, DNA fragmentation and cell death are biological processes which take place in a variety of cells involved in formation of teeth. The TUNEL staining technique is a simple but powerful tool to examine the fate of cells and tissues undergoing either programmed cell death (apoptosis) or fragmentation of nuclear DNA induced by external factors leading to pathological changes.
引用
收藏
页码:102 / 111
页数:10
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