Preparation and characterization of amnion hydrogel and its synergistic effect with adipose derived stem cells towards IL1β activated chondrocytes

被引：39

作者：

Bhattacharjee, Maumita ^{[1
,2
,3
]}

Ivirico, Jorge L. Escobar ^{[2
,3
,4
]}

Kan, Ho-Man ^{[1
,2
,3
]}

Bordett, Rosalie ^{[5
]}

Pandey, Rishikesh ^{[5
]}

Otsuka, Takayoshi ^{[1
,2
,3
]}

Nair, Lakshmi S. ^{[1
,2
,3
,6
,7
,8
,9
]}

Laurencin, Cato T. ^{[1
,2
,3
,4
,6
,7
,8
,9
]}

机构：

[1] Univ Connecticut Hlth, Connecticut Convergence Inst Translat Regenerat E, 263 Farmington Ave, Farmington, CT 06030 USA

[2] Univ Connecticut Hlth, Raymond & Beverly Sackler Ctr Biomed Biol Phys &, Farmington, CT 06030 USA

[3] Univ Connecticut Hlth, Dept Orthopaed Surg, Farmington, CT 06030 USA

[4] Univ Connecticut, Dept Chem & Biomol Engn, Storrs, CT 06269 USA

[5] Univ Connecticut Hlth, Sch Med, Connecticut Childrens Innovat Ctr, Farmington, CT USA

[6] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA

[7] Univ Connecticut, Dept Mat Sci & Engn, Storrs, CT 06269 USA

[8] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA

[9] Univ Connecticut Hlth, Sch Dent Med, Dept Craniofacial Sci, Farmington, CT 06030 USA

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

COLLAGEN FIBRILLOGENESIS; MATRIX; TISSUE; OSTEOARTHRITIS; CARTILAGE; MEMBRANE; INFLAMMATION; PATHOGENESIS; DECELLULARIZATION; PROLIFERATION;

D O I：

10.1038/s41598-020-75921-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Inflammation leads to chondrocyte senescence and cartilage degeneration, resulting in osteoarthritis (OA). Adipose-derived stem cells (ADSCs) exert paracrine effects protecting chondrocytes from degenerative changes. However, the lack of optimum delivery systems for ADSCs limits its use in the clinic. The use of extracellular matrix based injectable hydrogels has gained increased attention due to their unique properties. In the present study, we developed hydrogels from amnion tissue as a delivery system for ADSCs. We investigated the potential of amnion hydrogel to maintain ADSC functions, the synergistic effect of AM with ADSC in preventing the catabolic responses of inflammation in stimulated chondrocytes. We also investigated the role of Wnt/beta-catenin signaling pathway in IL-1 beta induced inflammation in chondrocytes and the ability of AM-ADSC to inhibit Wnt/beta-catenin signaling. Our results showed that AM hydrogels supported cell viability, proliferation, and stemness. ADSCs, AM hydrogels and AM-ADSCs inhibited the catabolic responses of IL-1 beta and inhibited the Wnt/beta-catenin signaling pathway, indicating possible involvement of Wnt/beta-catenin signaling pathways in IL-1 beta induced inflammation. The results also showed that the synergistic effect of AM-ADSCs was more pronounced in preventing catabolic responses in activated chondrocytes. In conclusion, we showed that AM hydrogels can be used as a potential carrier for ADSCs, and can be developed as a potential therapeutic agent for treating OA.

引用

页数：15

共 56 条

[51]

The Paracrine Effect of Adipose-Derived Stem Cells Inhibits IL-1β-induced Inflammation in Chondrogenic Cells through the Wnt/β-Catenin Signaling Pathway [J].