Genotoxicity of gliotoxin, a secondary metabolite of Aspergillus fumigatus, in a battery of short-term test systems

被引：44

作者：

Nieminen, SM

Mäki-Paakkanen, J

Hirvonen, MR

Roponen, M

von Wright, A

机构：

[1] Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland

[2] Natl Publ Hlth Inst, Dept Environm Hlth, FIN-70701 Kuopio, Finland

[3] Univ Kuopio, Inst Appl Biotechnol, FIN-70211 Kuopio, Finland

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2002年 / 520卷 / 1-2期

基金：

芬兰科学院;

关键词：

ghotoxin; Aspergillus fumigatus; indoor air; genotoxicity;

D O I：

10.1016/S1383-5718(02)00202-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genotoxic effects of gliotoxin, a known fungal secondary metabolite, were studied. Gliotoxin was purified from cultivation medium of Aspergillus fumigatus isolated from the indoor air of a moisture problem house. The genotoxicity of gliotoxin was assessed both in bacterial test systems including bacterial repair assay, Ames Salmonella assay and SOS-chromotest, and in mammalian cells using single cell gel (SCG) electrophoresis assay and sister-chromatid exchange (SCE) test. Gliotoxin was found to be genotoxic in the bacterial repair assay but, not in the Salmonella test or SOS-chromotest. A dose-related increase in DNA damage was observed in mouse RAW264.7 macrophages exposed to gliotoxin for 2 h in plain medium in the SCG assay. In contrast to the positive response in the SCG assay, gliotoxin did not induce any clear, dose-related increase in SCEs in Chinese hamster ovary (CHO) cells. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：161 / 170

页数：10

共 36 条

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