Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification

被引：23

作者：

Chen, Hao-tai ^{[1
]}

Zhang, Jie ^{[1
]}

Yang, Sheng-hai ^{[1
]}

Ma, Li-na ^{[1
]}

Ma, Yan-ping ^{[1
]}

Liu, Xiang-tao ^{[1
]}

Cai, Xue-peng ^{[1
]}

Zhang, Yong-guang ^{[1
]}

Liu, Yong-sheng ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Minist Agr,Key Lab Vet Publ Hlth,Key Lab Anim Vir, Lanzhou 730046, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 158卷 / 1-2期

关键词：

Porcine parvovirus (PPV); Detection; Loop-mediated isothermal amplification (LAMP); Sensitivity; Specificity; MULTISYSTEMIC WASTING SYNDROME; LINKED-IMMUNOSORBENT-ASSAY; RESPIRATORY SYNDROME VIRUS; DISEASE VIRUS; SYNDROME PMWS; PIGS; TRANSCRIPTION; CIRCOVIRUS-2; ORGANIZATION; ANTIBODIES;

D O I：

10.1016/j.jviromet.2009.02.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine parvovirus (PPV) DNA. The amplification could be finished in 45 min under isothermal condition at 62 degrees C by employing a set of four primers targeting VP2 gene of PPV. LAMP assay showed higher sensitivity than PCR, with a detection limit of 5 copies of PPV genomic DNA per reaction. No cross reactivity was observed from the samples of other related viruses including canine parvovirus, parvovirus B19, porcine circovirus type 1, porcine circovirus type 2 and porcine peudorabies virus. The detection rate of PPV LAMP for 125 clinical samples was 97.6% and appeared higher than that of PCR method. The result indicated the potential usefulness of the technique as a simple, rapid procedure for the detection of PPV. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：100 / 103

页数：4

共 27 条

[1] Development of a PCR-based method coupled with a microplate colorimetric assay for the detection of Porcine Parvovirus and application to diagnosis in piglet tissues and human plasma [J].