Direct measurement of protein adhesion at biomaterial surfaces by scanning force microscopy

被引：27

作者：

Vansteenkiste, SO

Corneillie, SI

Schacht, EH

Chen, X

Davies, MC

Moens, M

Van Vaeck, L

机构：

[1] Univ Ghent, Inst Biomed Technol, Dept Organ Chem, Polymer Mat Res Grp, B-9000 Ghent, Belgium

[2] Univ Nottingham, Dept Pharmaceut Sci, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England

[3] Univ Instelling Antwerp, Dept Chem, B-2610 Antwerp, Belgium

来源：

LANGMUIR | 2000年 / 16卷 / 07期

关键词：

D O I：

10.1021/la981740c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The adsorption of human serum albumin (HSA) onto tailor-made polyurethane biomaterial coatings was measured in a liquid environment by scanning force microscopy (SFM). The adhesion of HSA onto polyurethane films was probed by adhesion force measurements with protein-coated SFM tips. Results reveal that HSA adsorbs readily onto hydrophobic polyurethane surfaces. Adsorption time profiles of the HSA deposition were elucidated by dynamic in situ force-distance measurements. The introduction of poly(ethylene glycol) (PEG) grafts, present at the biomaterial interface, inhibited all interactions with HSA. Latter results were explained by the combined effects of steric repulsion forces, minimal interfacial free energy, and high chain mobility of the hydrated PEG grafts. These phenomena were in excellent agreement with measurements obtained in surface plasmon resonance experiments.

引用

页码：3330 / 3336

页数：7

共 50 条

[1]

ANDRADE JD, 1985, SURFACE INTERFACIAL, V2, P1

[2] Atomic (scanning) force microscopy in cardiovascular research [J].

Arnsdorf, MF ;

Xu, SH .

JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, 1996, 7 (07) :639-652

[3] SURFACE STUDIES BY SCANNING TUNNELING MICROSCOPY [J].

BINNING, G ;

ROHRER, H ;

GERBER, C ;

WEIBEL, E .

PHYSICAL REVIEW LETTERS, 1982, 49 (01) :57-61

[4]

Bottomley LA, 1996, ANAL CHEM, V68, pR185, DOI 10.1021/a1960008+

[5] MEASURING SURFACE FORCES IN AQUEOUS-ELECTROLYTE SOLUTION WITH THE ATOMIC-FORCE MICROSCOPE [J].

BUTT, HJ ;

JASCHKE, M ;

DUCKER, W .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1995, 38 (01) :191-201

[6] Force-distance curves by AFM - A powerful technique for studying surface interactions [J].

Capella, B ;

Baschieri, P ;

Frediani, C ;

Miccoli, P ;

Ascoli, C .

IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1997, 16 (02) :58-65

[7]

CARTER DC, 1994, ADV PROTEIN CHEM, V45, P153

[8] Recognition of protein adsorption onto polymer surfaces by scanning force microscopy and probe-surface adhesion measurements with protein-coated probes [J].

Chen, X ;

Davies, MC ;

Roberts, CJ ;

Tendler, SJB ;

Williams, PM ;

Davies, J ;

Dawkes, AC ;

Edwards, JC .

LANGMUIR, 1997, 13 (15) :4106-4111

[9] Application of protein-coated scanning force microscopy probes in measurements of surface affinity to protein adsorption [J].

Chen, X ;

Patel, N ;

Davies, MC ;

Roberts, CJ ;

Tendler, SJB ;

Williams, PM ;

Davies, J ;

Dawkes, AC ;

Edwards, JC .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 1998, 66 (Suppl 1) :S631-S634

[10]

CHRISTENSON HK, 1992, LONG RANGE ATTRACTIO

← 1 2 3 4 5 →