Electrochemical detector for microchip electrophoresis of poly(dimethylsiloxane) with a three-dimensional adjustor

被引：30

作者：

Bao, N ^{[1
]}

Xu, JJ ^{[1
]}

Dou, YH ^{[1
]}

Cai, Y ^{[1
]}

Chen, HY ^{[1
]}

Xia, XH ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Lab Life Analyt Chem, Nanjing 210093, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1041卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

electrochemical detection; detection; electrophoresis; chip technology; instrumentation; poly(dimethylsiloxane);

D O I：

10.1016/j.chroma.2004.04.036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper presents an electrochemical detector for poly(dimethylsiloxane) (PDMS) microchip CE with a three-dimensional adjustor which makes it possible to accurately align a separate working electrode that can be easily fabricated in laboratory to the uncertain PDMS microchannel outlet. The substantial influence of the electrode-PDMS microchannel distance was investigated. The optimal electrode-outlet distance was found to be 10 mum for the PDMS microchannel with the width of 50 mum due to its relatively slow electroosmotic flow. Adrenaline and catechol were well separated, with a linear response range from 20 muM to 1 mM, and a detection limit of 2 muM for catechol, using carbon disk electrode (diameter of 300 mum). Furthermore, arginine and histidine can be well separated and detected directly in the PDMS microchannel using a Cu disk electrode (diameter of 150 mum). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：245 / 248

页数：4

共 29 条

[1] Micro total analysis systems. 2. Analytical standard operations and applications
Auroux, PA
Iossifidis, D
Reyes, DR
Manz, A
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (12) : 2637 - 2652
[2] Fully integrated on-chip electrochemical detection for capillary electrophoresis in a microfabricated device
Baldwin, RP
Roussel, TJ
Crain, MM
Bathlagunda, V
Jackson, DJ
Gullapalli, J
Conklin, JA
Pai, R
Naber, JF
Walsh, KM
Keynton, RS
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (15) : 3690 - 3697
[3] Dou YH, 2002, ELECTROPHORESIS, V23, P3558, DOI 10.1002/1522-2683(200210)23:20<3558::AID-ELPS3558>3.0.CO
[4] 2-#
[5] Gawron AJ, 2001, ELECTROPHORESIS, V22, P242, DOI 10.1002/1522-2683(200101)22:2<242::AID-ELPS242>3.0.CO
[6] 2-W
[7] Electrochemical detectors prepared by electroless deposition for microfabricated electrophoresis chips
Hilmi, A
Luong, JHT
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (19) : 4677 - 4682
[8] Dynamic coating using polyelectrolyte multilayers for chemical control of electroosmotic flow in capillary electrophoresis microchips
Liu, Y
Fanguy, JC
Bledsoe, JM
Henry, CS
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (24) : 5939 - 5944
[9] Manica DR, 2002, ELECTROPHORESIS, V23, P3735, DOI 10.1002/1522-2683(200211)23:21<3735::AID-ELPS3735>3.0.CO
[10] 2-7

← 1 2 3 →