Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry

被引：78

作者：

Brivio, M ^{[1
]}

Fokkens, RH ^{[1
]}

Verboom, W ^{[1
]}

Reinhoudt, DN ^{[1
]}

Tas, NR ^{[1
]}

Goedbloed, M ^{[1
]}

van den Berg, A ^{[1
]}

机构：

[1] Univ Twente, Res Inst, MESA, Lab Supramol Chem & Technol, NL-7500 AE Enschede, Netherlands

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 16期

关键词：

D O I：

10.1021/ac020185n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A continuous flow micro total analysis system (mu-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the mu-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.

引用

页码：3972 / 3976

页数：5

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