Biochemical and genetic evidence for phospholipase C activity in Mycobacterium ulcerans

被引：14

作者：

Gomez, A

Mve-Obiang, A

Vray, B

Remacle, J

Chemlal, K

Meyers, WM

Portaels, F

Fonteyne, PA

机构：

[1] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium

[2] Free Univ Brussels, Fac Med, Expt Immunol Lab, Brussels, Belgium

[3] Fac Univ Notre Dame Paix, Lab Biochim & Biol Cellulaire, B-5000 Namur, Belgium

[4] Armed Forces Inst Pathol, Washington, DC 20306 USA

[5] Univ Quindio, Fac Med, Quinidio, Colombia

来源：

INFECTION AND IMMUNITY | 2000年 / 68卷 / 05期

关键词：

D O I：

10.1128/IAI.68.5.2995-2997.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.

引用

收藏

页码：2995 / 2997

页数：3

相关论文

共 25 条

[1]

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[2]

Emergence of a unique group of necrotizing mycobacterial diseases [J].

Dobos, KM ;

Quinn, FD ;

Ashford, DA ;

Horsburgh, CR ;

King, CH .

EMERGING INFECTIOUS DISEASES, 1999, 5 (03) :367-378

[3]

USE OF AN ORDERED COSMID LIBRARY TO DEDUCE THE GENOMIC ORGANIZATION OF MYCOBACTERIUM-LEPRAE [J].

EIGLMEIER, K ;

HONORE, N ;

WOODS, SA ;

CAUDRON, B ;

COLE, ST .

MOLECULAR MICROBIOLOGY, 1993, 7 (02) :197-206

[4]

Immunopathology of tuberculosis: Roles of macrophages and monocytes [J].

Fenton, MJ ;

Vermeulen, MW .

INFECTION AND IMMUNITY, 1996, 64 (03) :683-690

[5]

Bacterial phospholipase C upregulates matrix metalloproteinase expression by cultured epithelial cells [J].

Firth, JD ;

Putnins, EE ;

Larjava, H ;

Uitto, VJ .

INFECTION AND IMMUNITY, 1997, 65 (12) :4931-4936

[6]

Mycolactone:: A polyketide toxin from Mycobacterium ulcerans required for virulence [J].

George, KM ;

Chatterjee, D ;

Gunawardana, G ;

Welty, D ;

Hayman, J ;

Lee, R ;

Small, PLC .

SCIENCE, 1999, 283 (5403) :854-857

[7]

Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays [J].

Gordon, SV ;

Brosch, R ;

Billault, A ;

Garnier, T ;

Eiglmeier, K ;

Cole, ST .

MOLECULAR MICROBIOLOGY, 1999, 32 (03) :643-655

[8]

Comparison of two PCRs for detection of Mycobacterium ulcerans [J].

Guimaraes-Peres, A ;

Portaels, F ;

de Rijk, P ;

Fissette, K ;

Pattyn, SR ;

van Vooren, JP ;

Fonteyne, PA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) :206-208

[9]

INSERTION ELEMENT IS986 FROM MYCOBACTERIUM-TUBERCULOSIS - A USEFUL TOOL FOR DIAGNOSIS AND EPIDEMIOLOGY OF TUBERCULOSIS [J].

HERMANS, PWM ;

VANSOOLINGEN, D ;

DALE, JW ;

SCHUITEMA, ARJ ;

MCADAM, RA ;

CATTY, D ;

VANEMBDEN, JDA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) :2051-2058

[10]

FURTHER CHARACTERIZATION OF MYCOBACTERIUM-ULCERANS TOXIN [J].

HOCKMEYER, WT ;

KRIEG, RE ;

REICH, M ;

JOHNSON, RD .

INFECTION AND IMMUNITY, 1978, 21 (01) :124-128