Remote organ ischemic preconditioning protect brain from ischemic damage following asphyxial cardiac arrest

被引:83
作者
Dave, Kunjan R. [1 ]
Saul, Isabel [1 ]
Prado, Ricardo [1 ]
Busto, Raul [1 ]
Perez-Pinzon, Miguel A. [1 ]
机构
[1] Univ Miami, Miller Sch Med, Cerebral Vasc Dis Res Ctr, Dept Neurosci, Miami, FL 33101 USA
关键词
hippocampus; global cerebral ischemia; ischemic tolerance; neuroprotection; stroke; cell death;
D O I
10.1016/j.neulet.2006.05.037
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Ischemic preconditioning (IPC) is a phenomenon whereby an organ's adaptive transient resistance to a lethal ischemic insult occurs by preconditioning this organ with a sub-lethal/mild ischemic insult of short duration. Besides IPC, recent studies reported that a short sub-lethal ischemia and reperfusion in various organs can induce ischemic tolerance in another organ as well. This phenomenon is known as remote ischemic preconditioning (RPC). In the present study we tested the hypothesis that tolerance for ischemia can be induced in brain by RPC and IPC in a rat model of asphyxial cardiac arrest (ACA). RPC was induced by tightening the upper two-thirds of both hind limbs using a toumiquet for 15 or 30 min and IPC was induced by tightening bilateral carotid artery ligatures for 2 min. Eight minutes of ACA was induced 48 It after RPC or IPC. After 7 day of resuscitation, brains were extracted and examined for histopathological changes. In CA1 hippocampus, the number of normal neurons was 63% lower in cardiac-arrested rats as compared to the control group. The number of normal neurons in the 15 min RPC, 30 min RPC, and IPC groups was higher than the ACA group by 54, 70, and 67%, respectively. This study demonstrates that RPC and IPC are able to provide neuroprotection in a rat model of ACA. Besides direct application of RPC or IPC paradigms, the exploration of the mechanisms of observed neuroprotection by RPC and IPC may also lead to a possible therapy for CA patients. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:170 / 175
页数:6
相关论文
共 50 条
[31]   Sites of action of adenosine in interorgan preconditioning of the heart [J].
Liem, DA ;
Verdouw, PD ;
Ploeg, H ;
Kazim, S ;
Duncker, DJ .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2002, 283 (01) :H29-H37
[32]  
Malhotra S., 2005, J CEREB BLOOD FLOW M, V25, pS308
[33]   PRECONDITIONING WITH ISCHEMIA - A DELAY OF LETHAL CELL INJURY IN ISCHEMIC MYOCARDIUM [J].
MURRY, CE ;
JENNINGS, RB ;
REIMER, KA .
CIRCULATION, 1986, 74 (05) :1124-1136
[34]  
OXMAN T, 1997, AM J PHYSIOL-HEART C, V273, pH1707
[35]   Cardioprotection at a distance: Mesenteric artery occlusion protects the myocardium via an opioid sensitive mechanism [J].
Patel, HH ;
Moore, J ;
Hsu, AK ;
Gross, GJ .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2002, 34 (10) :1317-1323
[36]   Rapid preconditioning protects rats against ischemic neuronal damage after 3 but not 7 days of reperfusion following global cerebral ischemia [J].
PerezPinzon, MA ;
Xu, GP ;
Dietrich, WD ;
Rosenthal, M ;
Sick, TJ .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1997, 17 (02) :175-182
[37]   REGIONAL ISCHEMIC PRECONDITIONING PROTECTS REMOTE VIRGIN MYOCARDIUM FROM SUBSEQUENT SUSTAINED CORONARY-OCCLUSION [J].
PRZYKLENK, K ;
BAUER, B ;
OVIZE, M ;
KLONER, RA ;
WHITTAKER, P .
CIRCULATION, 1993, 87 (03) :893-899
[38]   εPKC is required for the induction of tolerance by ischemic and NMDA-mediated preconditioning in the organotypic hippocampal slice [J].
Raval, AP ;
Dave, KR ;
Mochly-Rosen, D ;
Sick, TJ ;
Pérez-Pinzón, MA .
JOURNAL OF NEUROSCIENCE, 2003, 23 (02) :384-391
[39]   TNF-α is required for late ischemic preconditioning but not for remote preconditioning of trauma [J].
Ren, XP ;
Wang, Y ;
Jones, WK .
JOURNAL OF SURGICAL RESEARCH, 2004, 121 (01) :120-129
[40]   Bradykinin mediates cardiac preconditioning at a distance [J].
Schoemaker, RG ;
van Heijningen, CL .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2000, 278 (05) :H1571-H1576