Genetic and physical mapping of the ovine cystic fibrosis gene

被引：6

作者：

Tebbutt, SJ ^{[1
]}

Broom, MF ^{[1
]}

vanStijn, TC ^{[1
]}

Montgomery, GW ^{[1
]}

Hill, DF ^{[1
]}

机构：

[1] UNIV OTAGO,CTR GENE RES,DUNEDIN,NEW ZEALAND

来源：

CYTOGENETICS AND CELL GENETICS | 1996年 / 74卷 / 04期

关键词：

CFTR; IDENTIFICATION; DNA;

D O I：

10.1159/000134425

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two yeast artificial chromosomes (YACs 48B6 and 88F7) that span the ovine cystic fibrosis transmembrane conductance regulator (CFTR) gene locus have been isolated. These YAC clones have been physically assigned to the interval 4q23 --> q25 on sheep chromosome 4, using fluorescence in situ hybridization. A sequence polymorphism in exon 7 of the CFTR gene was identified in Merino sheep and present at a low frequency. This polymorphism segregated in several flocks. Linkage analysis confirmed the location of CFTR on sheep chromosome 4. The most likely location is within a 13.7-cM region close to markers MAF70 and TGLA116.

引用

收藏

页码：245 / 247

页数：3

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